Hasim, Hidani and Kuttulebbai Naina Mohamed Salam, Sirajudeen and Rao, Pasupuleti Visweswara and Muthuraju, Sangu and Che Jalil, Nur Asyilla and Asari, Mohd Asnizam (2024) Effect of tualang honey-mediated silver nanoparticles on TNF-α level, caspase-3 activity and hippocampal morphology in Kainic Acid-induced neurodegeneration in male rats. IIUM Medical Journal Malaysia, 23 (4). pp. 92-100. ISSN 1823-4631 E-ISSN 2735-2285
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Abstract
INTRODUCTION: Despite being common disorder, the curative treatment for degenerative diseases are not yet available. Although Tualang honey (TH) has been reported to protect against neurodegeneration, but the effect of TH-mediated silver nanoparticles (THSN) on neurodegeneration is poorly understood. Thus, we conducted this study aimed to determine the effects of THSN on the levels of tumour necrosis factor alpha (TNF-α), caspase-3 activity, and hippocampal morphology in Kainic Acid (KA) induced neurodegeneration in rats. MATERIALS AND METHODS: A total of 72 Male Sprague Dawley rats were randomized into six groups which were the control, THSN 10mg, THSN 50 mg, KA only, KA+THSN 10 mg, and KA+THSN 50 mg groups. Each group was pre-treated orally with either distilled water or THSN (10 mg/kg or 50 mg/kg), according to their respective group. Following the last pre-treatment, each rat was injected with KA (15 mg/kg) or saline. After 24 h and 5 days of KA induction, all rats were sacrificed, and the hippocampus from each rat was harvested. Cresyl Violet and Fluoro Jade C staining were carried out to examine the number of viable cells and degenerating neurons. TNF-α level and caspase-3 activity in the hippocampus were measured using commercially available ELISA kits. RESULTS: Rats with KA-induced neurodegeneration demonstrated a significant increase (p<0.05) of TNF-α level and caspase-3 activity with a lower number of viable cells and increased number of degenerating neurons in the hippocampus. The pre-treatments of THSN groups improved these changes by lowering the TNF-α level and caspase-3 activity and decreasing the number of degenerating neurons. CONCLUSION: THSN could have potential neuroprotective effects in ameliorating TNF-α level, caspase-3 activity, and hippocampal damage in KA-induced male rats.
| Item Type: | Article (Journal) |
|---|---|
| Additional Information: | 9547/114779 |
| Uncontrolled Keywords: | Tualang Honey, Silver Nanoparticles, TNF-α, Caspase-3, Rats’ Hippocampus |
| Subjects: | R Medicine > R Medicine (General) |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Medicine > Department of Basic Medical Kulliyyah of Medicine |
| Depositing User: | Prof.Dr. SIRAJUDEEN KUTTULEBBAI NAINAMOHAMED SALAM |
| Date Deposited: | 01 Oct 2024 16:33 |
| Last Modified: | 01 Oct 2024 16:51 |
| URI: | http://irep.iium.edu.my/id/eprint/114779 |
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