Characterizing the adult zebrafish model of Parkinson’s disease: a systematic review of dynamic changes in behavior and physiology post-MPTP administration

Razali, Khairiah and Kumar, Jaya and Mohamed, Wael Mohamed Yousef (2024) Characterizing the adult zebrafish model of Parkinson’s disease: a systematic review of dynamic changes in behavior and physiology post-MPTP administration. Frontiers in Neuroscience, 18. ISSN 1662-4548 E-ISSN 1662-453X

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Abstract

Introduction: Adult zebrafish are increasingly used in Parkinson’s disease (PD) research due to their well-characterized dopaminergic system. Among the toxin-based models, the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) is widely utilized to induce parkinsonism in adult zebrafish. Therefore, this review presents an overview of the procedures and the dynamic changes in behavior and physiology observed in the adult zebrafish PD model following a single intraperitoneal injection of MPTP. Methods: A systematic literature search in the PubMed and Google Scholar databases was conducted to identify relevant articles. Of the 165 articles identified, 9 were included in this review. These chosen articles are original works published before March 2024, all of which utilized adult zebrafish induced with MPTP as the model for PD. Other articles were excluded based on factors such as limited relevance, utilization of zebrafish embryos or larvae instead of adults, and variations in MPTP deliveries. Results: Studies indicated that the ideal model entails the utilization of mixed gender zebrafish aged between 4 and 6 months from the wild-type strain. The acceptable MPTP doses ranges between 20 μg/g (lowest) and 225 μg/g (highest) and doses above 292 μg/g are lethal. Furthermore, noticeable parkinsonian symptoms appear 1 day after administration and persist for more than 1 week. Discussion: Mitochondrial dysfunction precedes dopaminergic neurodegeneration within this experimental regime. A single administration of MPTP effectively induces PD in adult zebrafish. This study aids in crafting the adult zebrafish PD model, outlining the progressive behavioral and physiological changes ensuing from MPTP administration.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Parkinson’s disease, zebrafish, MPTP, intraperitoneal injection, mitochondrial dysfunction
Subjects:	R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine Kulliyyah of Medicine > Department of Basic Medical
Depositing User:	Dr Wael Mohamed
Date Deposited:	11 Sep 2024 08:30
Last Modified:	07 Oct 2024 14:03
URI:	http://irep.iium.edu.my/id/eprint/114376

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