Zainuddin, Nor'Izzah and Mohd Zaini Makhtar, Muaz and Nagoor Gunny, Ahmad Anas and Subash Chandra Bose, Gopinath and Ahmad, Abdul Aziz and Pusphanathan, Kavita and Siddiqui, Masoom Raza and Alam, Mahboob and Mohd, Rafatullah (2024) Isolation, screening and optimization of alkaliphilic cellulolytic fungi for production of cellulase. Green Processing and Synthesis, 13 (1). pp. 1-16. ISSN 2191-9550
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Abstract
This study concerns with the production and partial characterization of alkaline cellulase from alkaliphilic cellulolytic (AC) fungi isolated fromsoil in Perlis, Malaysia. The best fungi strain was selected on the basis of producing the highest cellulase at high pH conditions. Cellulase from the selected fungi strain was further characterized under saccharification but varies in operating parameters. Finally, the kineticmodel describing the growth of the AC fungi strain was studied by employing the logistic model. Among the tested fungi strains, Basidiomycetes strain (BK1) showed high potentiality for the production of maximum alkaline cellulase production at pH 9 after 72 h of incubation at 30°C containing 6 g·L−1 carboxyl methyl cellulose. The saccharification process showed that the enzyme favour high alkaline condition and proves thermotolerant properties, while 15% (v/v) enzyme loading and 1% substrate concentration recorded the highest glucose production at about 1.2–1.3mg·mL−1. The novelty of the study is to identify and optimize a unique indigenous fungi that emit alkaliphilic cellulase as alternative usage in biotechnology industries due to its capacity to adapt to the extreme conditions of specific industrial processes. There are revolutionary options for use in biotechnological businesses that involve high pH and therefore have substantial biotechnological promise.
| Item Type: | Article (Journal) |
|---|---|
| Uncontrolled Keywords: | alkaliphilic cellulolytic fungi, alkaline cellulase, enzyme, saccharification, logistic growth model |
| Subjects: | Q Science > Q Science (General) Q Science > QD Chemistry Q Science > QR Microbiology |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Engineering > Department of Science Kulliyyah of Engineering |
| Depositing User: | Asst. Prof Abdul Aziz Ahmad |
| Date Deposited: | 25 Jul 2024 15:14 |
| Last Modified: | 25 Jul 2024 15:14 |
| URI: | http://irep.iium.edu.my/id/eprint/113298 |
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