Physicochemical and sensory attributes of Trihoney blend (Trigona sp., Apis mellifera, and Apis dorsata) for enhanced antioxidant optimization using Response surface methodology (RSM)

Abdul Rafa, Ainin Azwani and Ibrahim, Muhammad and Zakaria, Nor Hafizah and Anuar, Mohd Nur Nasyriq and Mat Alewi, Nur Aizura and Abdul Ghani, Radiah and Abdul Majid, Fadzilah Adibah (2024) Physicochemical and sensory attributes of Trihoney blend (Trigona sp., Apis mellifera, and Apis dorsata) for enhanced antioxidant optimization using Response surface methodology (RSM). IIUM Medical Journal Malaysia, 23 (3). pp. 38-45. ISSN 1823-4631 E-ISSN 2735-2285

PDF - Published Version Restricted to Registered users only Download (1MB) | Request a copy

Abstract

NTRODUCTION: The therapeutic applications of honey products have been extensively studied, but the various combination of honeys as a high-antioxidant product has not been explored. This study aimed to develop an optimized three honey formulation (Trihoney) with maximal antioxidant potency and physicochemical characteristics, as well as favourable among panellists. MATERIALS AND METHODS: The three types of honey studied are; i) Trigona sp. honey (TH), ii) Apis mellifera honey (MH), and iii) Apis dorsata honey (DH). Response surface methodology (RSM) was employed to design optimal Trihoney formulation for i) total phenolic content (TPC), ii) ferric-reducing ability of plasma (FRAP), and iii) 2,2’-diphenyl-1 picrylhydrazyl (DPPH) radical scavenging activity. Proximate and nutrient analyses, such as moisture, protein, fibre, carbohydrate, sugar, and gross energy were also conducted. Additionally, sensory evaluation was carried out to support the findings. RESULTS: Based on RSM, three optimal Trihoney formulation were developed; i) Trihoney 1 (MH15:DH10:TH45), ii) Trihoney 2 (MH15:DH10:TH25), and iii) Trihoney 3 (MH15:DH10:TH15). Trihoney 1 was the most promising formulation, exhibiting the highest TPC (0.50 mg/GAE/g), remarkable values of FRAP (230.85 AAE/ g) and DPPH (86.32%). Physicochemical and sugar analysis indicated that all values complied with permitted quality standards. MH received the highest overall acceptability scores based on sensory evaluation. CONCLUSION: These findings warrant further extensive investigation of Trihoney formulation in animal studies to support its efficacy a valuable food supplement

Item Type:	Article (Journal)
Uncontrolled Keywords:	Antioxidant capacity, Response surface methodology, Sensory evaluation, Stingless bee, Trihoney
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Allied Health Sciences > Department of Nutrition Sciences
Depositing User:	Dr. Radiah Abdul Ghani
Date Deposited:	15 Jul 2024 12:08
Last Modified:	15 Jul 2024 12:08
URI:	http://irep.iium.edu.my/id/eprint/113182

Actions (login required)

View Item