Effect of pH, temperature and agitation on thermophilic biohydrogen production using immobilized cells on carbon composites (GAC-NiFe3O4)

Engliman, Nurul Sakinah and Jamali, Nur Syakina (2024) Effect of pH, temperature and agitation on thermophilic biohydrogen production using immobilized cells on carbon composites (GAC-NiFe3O4). Chemical Engineering Journal, 485. pp. 1-11. ISSN 1385-8947

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Abstract

Carbon composites-immobilized cell was utilized to enhance biohydrogen production. The initial pH, temperature and agitation effect was studied using the one-factor-at-a-time (OFAT) method. The optimal initial pH obtained was at pH 6.0 with hydrogen yield (HY) of 2.66 ± 0.09 mol H2/mol sugar, correlating with Gompertz constant of Hm = 974.99 mL, Rm = 17.90 mL/h and λ = 1.70 h. At 60 ◦C, the highest HY was obtained at 2.75 ± 0.11 mol H2/mol sugar. These corresponded to the Gompertz constant of Hm = 934.58 mL, Rm = 18.78 mL/h and λ = 1.89 h. The optimal agitation obtained was at 120 rpm, attaining the highest HY of 3.44 ± 0.54 mol H2/mol sugar, corresponding to the Gompertz constant of Hm = 1054.86 mL, Rm = 24.37 mL/h and λ = 3.90 h. The rRNA sequencing result revealed that the predominant species in the study was Thermoanaerobacterium. This study provides critical insight into process control conditions on biohydrogen production.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Thermophilic biohydrogen production Dark fermentation Granular activated carbon nanocomposites Immobilized cell Process parameter
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Nurul Sakinah Engliman
Date Deposited:	02 Jul 2024 15:39
Last Modified:	02 Jul 2024 15:47
URI:	http://irep.iium.edu.my/id/eprint/112888

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