Hepatic and renal effects of oral stingless bee honey in a streptozotocin-induced diabetic rat model

Mohd Nasir, Suriati and Ismail, Anis Farihan and Tuan Ismail, Tuan Salwani and Wan Abdul Rahman, Wan Faiziah and Wan Ahmad, Wan Amir Nizam and Tengku Din, Tengku Ahmad Damitri Al-Astani and Kuttulebbai Naina Mohamed Salam, Sirajudeen (2024) Hepatic and renal effects of oral stingless bee honey in a streptozotocin-induced diabetic rat model. World Journal of Experimental Medicine, 14 (1). E-ISSN 2220-315X

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Abstract

BACKGROUND Diabetes is known damage the liver and kidney, leading to hepatic dysfunction and kidney failure. Honey is believed to help in lowering the blood glucose levels of diabetic patients and reducing diabetic complications. However, the effect of stingless bee honey (SBH) administration in relieving liver and kidney damage in diabetes has not been well-studied. AIM To investigate the effect of SBH administration on the kidney and liver of streptozotocin-induced (STZ; 55 mg/kg) diabetic Sprague Dawley rats. METHODS The rats were grouped as follows (n = 6 per group): non-diabetic (ND), untreated diabetic (UNT), metformin-treated (MET), and SBH+metformin-treated (SBME) groups. After successful diabetic induction, ND and UNT rats were given normal saline, whereas the treatment groups received SBH (2.0 g/kg and/or metformin (250 mg/kg) for 12 d. Serum biochemical parameters and histological changes using hematoxylin and eosin (H&E) and periodic acid–Schiff (PAS) staining were evaluated. RESULTS On H&E and PAS staining, the ND group showed normal architecture and cellularity of Bowman’s capsule and tubules, whereas the UNT and MET groups had an increased glomerular cellularity and thickened basement membrane. The SBH-treated group showed a decrease in hydropic changes and mild cellularity of the glomerulus vs the ND group based on H&E staining, but the two were similar on PAS staining. Likewise, the SBME-treated group had an increase in cellularity of the glomerulus on H&E staining, but it was comparable to the SBH and ND groups on PAS staining. UNT diabetic rats had tubular hydropic tubules, which were smaller than other groups.Reduced fatty vacuole formation and dilated blood sinusoids in liver tissue were seen in the SBH group. Conversely, the UNT group had high glucose levels, which subsequently increased MDA levels, ultimately leading to liver damage. SBH treatment reduced this damage, as evidenced by having the lowest fasting glucose, serum alanine transaminase, aspartate transaminase, and alkaline phosphatase levels compared to other groups, although the levels of liver enzymes were not statistically significant. CONCLUSION The cellularity of the Bowman’s capsule, as well as histological alteration of kidney tubules, glomerular membranes, and liver tissues in diabetic rats after oral SBH resembled those of ND rats. Therefore, SBH exhibited a protective hepatorenal effect in a diabetic rat model.

Item Type:	Article (Journal)
Additional Information:	9547/111782
Uncontrolled Keywords:	Diabetes, Streptozotocin, Stingless bee honey, Hematoxylin and eosin, Periodic acid–Schiff, Liver, Kidney
Subjects:	R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine Kulliyyah of Medicine > Department of Basic Medical
Depositing User:	Prof.Dr. SIRAJUDEEN KUTTULEBBAI NAINAMOHAMED SALAM
Date Deposited:	08 Apr 2024 14:05
Last Modified:	13 Sep 2024 17:26
URI:	http://irep.iium.edu.my/id/eprint/111782

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