Qaralleh, Haitham and Mohammed Saghir, Sultan Ayesh and Al-limoun, Muhamad Odeh Atyeh and M. Dmor, Saif and Khleifat, Khaled and Mustafa Al-Ahmad, Basma Ezzat and Al-Omari, Laila and Tabana, Yasser and Mothana, Ramzi Ahmed and M. Al-Yousef, Hanan and M. Alqahtani, Abdulaziz (2024) Effect of Matricaria aurea essential oils on biofilm development, virulence factors and quorum sensing-dependent genes of Pseudomonas aeruginosa. Pharmaceuticals, 17 (3). ISSN 1424-8247
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Abstract
Abstract: The emergence of drug-resistant microorganisms presents a substantial global public health threat. The increase in pathogens resistant to commonly prescribed antibiotics underscores the urgent requirement to explore alternative treatment strategies. This study adopts a novel approach by harnessing natural resources, specifically essential oils (EO), to combat bacterial pathogenicity. The primary aim of this research was to analyze the chemical composition of the aerial part of the Matricaria aurea (M. aureas) EO and evaluate its potential for inhibiting quorum sensing (QS) and disrupting biofilm formation in Pseudomonas aeruginosa (P. aeruginosa). The gas chromatography-mass spectrometry (GCMS) analysis unveiled that α-bisabolol oxide A constituted the predominant portion, comprising 64.8% of the total, with β-bisabolene at 6.3% and α-farnesene at 4.8% following closely behind. The antibiofilm efficacy was observed at concentrations of 0.3, 0.15, and 0.08 mg/mL, demonstrating negligible effects on cell viability. Furthermore, the EO from M. aurea effectively inhibited the formation of P. aeruginosa biofilms by diminishing aggregation, hydrophobicity, and swarming motility. Significantly, the EO treatment resulted in a conspicuous decrease in the production of pyocyanin, rhamnolipid, and extracellular polymeric substances (EPS), along with a reduction in the enzymatic activity of protease and chitinase. The EO effectively hindered QS by disrupting QS mechanisms, resulting in a marked decline in the secretion of N-Acyl homoserine lactone (AHL) molecules and the expression of phazA1 and aprA genes. This investigation offers compelling evidence supporting the potential of M. aurea EO as a promising therapeutic candidate for addressing infectious diseases induced by biofilm formation.
| Item Type: | Article (Journal) |
|---|---|
| Additional Information: | 5816/111654 |
| Uncontrolled Keywords: | quorum sensing, biofilm, Matricaria aurea, α-bisabolol oxide A, Pseudomonas aeruginosa, virulence factors, phazA1, aprA |
| Subjects: | R Medicine > R Medicine (General) |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Dentistry Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences |
| Depositing User: | Dr Basma Al-Ahmad |
| Date Deposited: | 27 Mar 2024 14:35 |
| Last Modified: | 27 Mar 2024 14:37 |
| URI: | http://irep.iium.edu.my/id/eprint/111654 |
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