Funtional characterization of putative late hypocotyl (LHY) gene in stevia rebaudiana MS007 in silico analysis

Samsulrizal, Nurul Hidayah and Abdul Rahim, Zabirah and Mohamad Nazar, Siti Noor Eliana and Mustafa, Nur Farhana (2023) Funtional characterization of putative late hypocotyl (LHY) gene in stevia rebaudiana MS007 in silico analysis. Chemical and Natural Resources Engineering Journal, 7 (2). pp. 1-12. E-ISSN 2637-0719

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Abstract

Stevia rebaudiana is a medicinal herb that has been known as a low-calorie sweetener. It has been recognised as an artificial sweetener that is 300 times sweeter than sucrose. It is a commercially important sweetener that has been consumed as food and medicine, due to its ability to reduce blood sugar levels. Stevioside is one of the important compounds that give food a sweet taste. Previous studies showed that high amount of stevioside could be produced by delaying the flowering process of stevia. The aim of this study is to characterise the putative Late Elongated Hypocotyl (LHY) gene that is involved in the flowering process by using bioinformatics tools. Both analyses by using BLAST and domain search found that LHY gene is involved in the flowering process. These domains are SANT/Myb domain (IPR001005/SM000717), TEA domain (SM00426), Myb domain (IPR017930), and Myb domain plants (IPR006447). The phylogenetic tree was built using 20 out of 100 protein sequences from BLAST. The physico-chemical properties of putative LHY protein had been conducted through ProtParam and TMHMM, which showed that the protein is a globular protein. The phylogenetic tree construction indicated the common ancestor for the putative LHY protein, which is from the same family, i.e., Asteraceae. In summary, this study improves our knowledge of Stevia MS007 LHY gene by in-silico analysis. Therefore, future research should focus on determining the precise function of the protein in regulating the blooming stage of the stevia plant.

Item Type:	Article (Journal)
Uncontrolled Keywords:	putative late hypocotyl (LHY) gene
Subjects:	S Agriculture > S Agriculture (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science Kulliyyah of Science > Department of Plant Science
Depositing User:	Dr Nurul Hidayah Samsulrizal
Date Deposited:	22 Jan 2024 14:21
Last Modified:	09 Mar 2024 16:04
URI:	http://irep.iium.edu.my/id/eprint/110413

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