The cytotoxic effect of Cananga odorata (CO) essential oil on skin cancer is not via TP53 gene expression modulation

Anuar, Muhammad Ashraf Fithri (2023) The cytotoxic effect of Cananga odorata (CO) essential oil on skin cancer is not via TP53 gene expression modulation. Medicine & Health.

Preview	PDF (Abstract book) - Published Version Download (375kB) | Preview
	PDF (Certificate) - Supplemental Material Restricted to Repository staff only Download (1MB)

Abstract

Introduction: Skin cancer is one of the most common cancers worldwide. Cananga odorata (CO) essential oil (EO) has been reported to have anticancer and antioxidant properties. Hence, it may have the potential to be used as a topical treatment for skin cancer. The aim of this study is to assess the cytotoxic effect of CO EO on skin cancer, the pro-apoptotic TP53 gene expression, and its protein (p53) secretion. Materials and Methods: A431 squamous skin cancer cells and HFF1 fibroblast cells were treated with CO EO at 125, 250, and 500 μg/ml for 24 h. Their morphology was observed at 20-40x magnification. TP53 gene expression in these cells was examined by real-time PCR. p53 protein secretion in the conditioned media was assessed using western blot. Results: CO EO treated A431 cells exhibited loss of cellular margin and higher non-viable cells at all concentrations. Meanwhile, HFF1 cells treated with 125 μg/ml CO EO showed no significant changes. At 250 and 500 μg/ml, there was no significant changes in TP53 gene expression in A431 cells, but higher p53 protein secretion was observed. Conclusion: Our findings suggest that CO EO was cytotoxic to skin cancer at 250 and 500 μg/ml without augmenting TP53 gene expression. Hence, further investigations into CO EO effect on other apoptotic or anti-proliferative regulatory genes are required to establish its underlying molecular mechanism leading to its cytotoxic effect in skin cancer.

Item Type:	Article (other)
Uncontrolled Keywords:	Carcinogenesis, flower essences, gene expression regulation, post- transcriptional RNA processing, ylang ylang
Subjects:	Q Science > QP Physiology R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	UNSPECIFIED
Depositing User:	Dr Wan Fatein Nabeila Wan Omar
Date Deposited:	02 Jan 2024 09:40
Last Modified:	24 Jan 2024 23:33
URI:	http://irep.iium.edu.my/id/eprint/109648

Actions (login required)

View Item