Promoting cardioprotection with fenugreek: Insights from CoCl2 -induced hypoxia in neonatal rat cardiomyocytes

Hanafi, Noorul Izzati and Mohamed, Maizan and Kuttulebbai Naina Mohamed Salam, Sirajudeen and Saidan, Noor Hafizoh and Hua, Gan Siew and Abdul Kadir Pahirulzaman, Khomaizon and Rao, Pasupuleti Visweswara (2023) Promoting cardioprotection with fenugreek: Insights from CoCl2 -induced hypoxia in neonatal rat cardiomyocytes. Iranian Journal of Basic Medical Sciences, 26 (11). pp. 1360-1369. ISSN 2008-3866 E-ISSN 2008-3874

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Abstract

Objective(s): This study aimed to investigate the protective effects of fenugreek on CoCl2 -induced hypoxia in neonatal rat cardiomyocytes. Materials and Methods: Primary cardiomyocytes were isolated from Sprague Dawley rats aged 0–2 days and incubated with various concentrations of fenugreek (10-320 µg/ml) and CoCl2 -induced hypoxia for different durations (24, 48, and 72 hr). Cell viability, calcium signaling, beating rate, and gene expression were evaluated. Results: Fenugreek treatments did not cause any toxicity in cardiomyocytes. At a concentration of 160 µg/ml for 24 hr, fenugreek protected the heart against CoCl2 -induced hypoxia, as evidenced by reduced expression of caspases (-3, -6, -8, and -9) and other functional genes markers, such as HIF-1α, Bcl-2, IP3R, ERK5, and GLP-1r. Calcium signaling and beating rate were also improved in fenugreektreated cardiomyocytes. In contrast, CoCl2 treatment resulted in up-regulation of the hypoxia gene HIF-1α and apoptotic caspases gene (-3, -9, -8, -12), and down-regulation of Bcl-2 activity. Conclusion: Fenugreek treatment at a concentration of 160 µg/ml was not toxic to neonatal rat cardiomyocytes and protected against CoCl2 -induced hypoxia. Furthermore, fenugreek improved calcium signaling and beating rate and altered gene expression. Fenugreek may be a potential therapeutic agent for promoting cardioprotection against hypoxia-induced injuries

Item Type:	Article (Journal)
Uncontrolled Keywords:	Cardiomyocytes Hypoxia Ischemia Therapeutics Trigonella foenum-graecum
Subjects:	R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine Kulliyyah of Medicine > Department of Basic Medical
Depositing User:	Prof.Dr. SIRAJUDEEN KUTTULEBBAI NAINAMOHAMED SALAM
Date Deposited:	09 Nov 2023 11:03
Last Modified:	23 Jan 2024 09:58
URI:	http://irep.iium.edu.my/id/eprint/108002

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