Detection of tanB gene Streptococcus gallolyticus subspecies gallolyticus in stool of colorectal cancer patients

Ismail, Che Muhammad Khairul Hisyam and Hamzah, Hairul Aini and Mohammad Aidid, Edre and Shalihin, Mohd Shaiful Ehsan and Md Nor, Azmi (2023) Detection of tanB gene Streptococcus gallolyticus subspecies gallolyticus in stool of colorectal cancer patients. Asia Pasific Journal of Molecular Biology & Biotechnology, 31 (2 (Suppl.)). p. 101. ISSN 0128-7451 E-ISSN 2672-7277

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Abstract

Background: tanB gene encodes for tannase and is specific for Streptococcus gallolyticus subspecies gallolyticus (SGG). The human gut may be colonized by SGG through zoonotic transmission. Patients with colorectal carcinoma (CRC) have a higher SGG colonization rate than healthy humans. Therefore, the detection of tanB SGG in patients with early-stage CRC might be a useful screening tool. We aimed to establish a stool PCR technique without the need for bacterial cultures. Method: stool samples were collected from 113 patients, who were referred to SASMEC@IIUM health centre for colonoscopy. Bacterial DNA was directly extracted from the stool specimen before PCR. The PCR optimisation involved two pairs of primers targeting the tanB gene. DNA sequences of random samples were deduced by DNA sequencing and further analysed to determine the analytical specificity. The sensitivity of the technique was determined by a 10-fold dilution of a known standard. Results: Thirty-three (33) out of 113 patients were diagnosed with CRC. Upon optimization with two CRC samples, primer pair 1 showed a high analytical specificity of the PCR. The sensitivity of the assay reached 102 DNA copies/ml. As expected, further testing on all samples resulted in a higher rate of SGG detection in CRC samples (50%; n=16/33) compared to the non-CRC samples (20%; n=16/80). Conclusion: The established PCR technique potentially benefits early CRC diagnosis.

Item Type:	Article (other)
Uncontrolled Keywords:	Streptococcus gallolyticus, bacteria, polymerase chain reaction, gene, colorectal cancer
Subjects:	Q Science > QR Microbiology R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine Kulliyyah of Medicine > Department of Basic Medical Kulliyyah of Medicine > Department of Community Medicine (Effective: 1st January 2011) Kulliyyah of Medicine > Department of Family Medicine (Effective: 1st January 2011) Kulliyyah of Medicine > Department of Surgery Kulliyyah of Science
Depositing User:	Dr Hairul Aini Hamzah
Date Deposited:	07 Sep 2023 16:56
Last Modified:	07 Sep 2023 16:57
URI:	http://irep.iium.edu.my/id/eprint/105219

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