Electrospun sulfonatocalix[4]arene loaded blended nanofibers: process optimization and In Vitro studies

Wan Abdul Khodir, Wan Khartini and Abd Hamid, Shafida and Yusof, Mohd Reusmaazran and Cruz-Maya, Iriczalli and Guarino, Vincenzo (2022) Electrospun sulfonatocalix[4]arene loaded blended nanofibers: process optimization and In Vitro studies. Pharmaceutics, 14 (9). pp. 1-16. ISSN 1999-4923

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Abstract

In the past decade, electrospun nanofibers made of biodegradable polymers have been used for different biomedical applications due to their flexible features in terms of surface area to volume ratio, pores, and fiber size, as well as their highly tunable surface properties. Recently, interest is growing in the use of supramolecular structures in combination with electrospun nanofibers for the fabrication of bioactive platforms with improved in vitro responses, to be used for innovative therapeutic treatments. Herein, sulfonatocalix[4]arene (SCX4) was synthesized from ptert-butyl-calix[4]arene and embedded in electrospun nanofibers made of polycaprolactone (PCL) and gelatin (GEL). The supramolecular structure of SCX4 and its efficient entrapment into electrospun fibers was confirmed by NMR spectroscopy and FTIR analysis, respectively. SEM analysis supported via image analysis enabled the investigation of the fiber morphology at the submicrometric scale, showing a drastic reduction in fiber diameters in the presence of SCX4: 267 ± 14 nm (without SCX) to 115 ± 5 nm (3% SCX4). Moreover, it was demonstrated that SCX4 significantly contributes to the hydrophilic properties of the fiber surface, as was confirmed by the reduction in contact angles from 54 ± 1.4° to 31 ± 5.5° as the SCX4 amount increased, while no effects on thermal stability were recognized, as was confirmed by TGA analyses. In vitro tests also confirmed that SCX4 is not cytotoxic, but plays a supporting role in L929 interactions, as was validated by the cell viability of PGC15% after 7 days, with respect to the control. These preliminary but promising data suggest their use for the fabrication of innovative platforms able to bind SCX4 to bioactive compounds and molecules for different therapeutic applications, from molecular recognition to controlled drug delivery

Item Type:	Article (Journal)
Uncontrolled Keywords:	sulfonatocalixarene; electrospun fibers; polycaprolactone; gelatin; in vitro
Subjects:	Q Science > QD Chemistry R Medicine > RS Pharmacy and materia medica > RS192 Materia Medica-Pharmaceutical Technology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Chemistry Kulliyyah of Science
Depositing User:	Dr Shafida Abd Hamid
Date Deposited:	29 Nov 2022 16:15
Last Modified:	29 Nov 2022 16:28
URI:	http://irep.iium.edu.my/id/eprint/101469

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