Abdur Rashid, Mohammad and Sarker, Md. Zaidul Islam and Ahmed, Qamar Uddin and Ferdosh, Sahena and Helal Uddin, A.B.M. (2021) In vitro α-glucosidase inhibitory activity of Mahkota Dewa fruit flesh using different extraction methods. In: International conference of Intigrative Medicine, 17-19 August 2021, USM, Penang. (Unpublished)
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Abstract
Purpose: Before using any plant for medicinal purposes, the proper extraction method must be considered for the bioactive compounds, which can be either polar or non-polar or both, and its advantages and disadvantages, depending on the selection process. The aim of this research was to compare the α-glucosidase inhibitory activity of Mahkota dewa fruit flesh using conventional and non-conventional extraction techniques. Method: The extraction was performed by heat reflex (HR), supercritical fluid extraction (SFE), and subcritical carbon dioxide (Sub-CO2) extraction using different temperatures, pressure, solvent ratio, flow rate. The optimized extracts were analyzed for a-glucosidase inhibitory activity with Saccharomyces cerevisiae enzyme. Result: The yields and α-glucosidase inhibitory activity of the Mahkota dewa extracts were analyzed. The yield was obtained in the order of HR>SubCO2>SFE on the other hand the IC50 value of α- glucosidase inhibitory activity was estimated in the order of SubCO2> HR >SFE due to its mild operating temperature and relatively low-pressure condition. Because carbon dioxide is a non- polar solvent that prefers mostly non-polar compounds, adding a co- solvent increases its polarity and improves the ability to extract more polar compounds. Furthermore, after depressurization, carbon dioxide can be easily separated from the end product. As a result, SubCO2 is the most practical extraction method for achieving higher quality of extract. Conclusion: The composition and bioactivities of extracts of natural products were typically influenced by the extracting method, solvent, and temperature.
| Item Type: | Conference or Workshop Item (Invited Papers) |
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| Subjects: | Q Science > QD Chemistry R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry |
| Depositing User: | Dr. A. B. M. Helal Uddin |
| Date Deposited: | 02 Dec 2022 11:11 |
| Last Modified: | 02 Dec 2022 11:11 |
| URI: | http://irep.iium.edu.my/id/eprint/101334 |
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