Bioautography, combination effects and photo-activated enzymatic restriction inhibitory activity of antimicrobial alkaloids from Glycosmis pentaphylla (Retz.) DC.

Md Taib, Nurhaya and Mohd Hassan, Norazian and Mohd Kamal, Laina Zarisa and Khin Soe, May (2022) Bioautography, combination effects and photo-activated enzymatic restriction inhibitory activity of antimicrobial alkaloids from Glycosmis pentaphylla (Retz.) DC. Malaysian Journal of Science, 41 (3). pp. 1-9. ISSN 1394-3065 E-ISSN 2600-8688

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Abstract

Glycosmis pentaphylla (Retz.) DC., locally known as nerapan, has long been used in Asian countries as a traditional remedy for ailments attributed to microbial infections. This study aims to isolate antimicrobial alkaloids from G. pentaphylla, to determine their combination effects with selected antimicrobial agents and to screen for their photoactivated enzymatic restriction inhibitory activity. Bioautography-guided isolation of antimicrobial alkaloids was performed by using column chromatography with Staphylococcus aureus, Escherichia coli, and Candida albicans as the indicator microbes. The antimicrobial effects of the alkaloids combined with selected antimicrobial agents, namely, ciprofloxacin, erythromycin, vancomycin, and ketoconazole, were determined by using a checkerboard assay. Photoactivated enzymatic restriction inhibitory activity was assessed by using agarose gel electrophoresis. Two antimicrobial active alkaloids were isolated and identified as arborinine and arborine. The antimicrobial activity of arborinine and arborine was determined to be in the range of 250 μg/ml and 1000 μg/ml. Partial synergy was observed for all arborine-antibiotics and arborinine-ketoconazole interactions against S. aureus and C. albicans, respectively. Arborine was relatively the strongest photoactivated enzymatic restriction inhibitor, particularly against EcoRI, PstI, and SalI. The results obtained are promising and encourage further research on the alkaloids as potential antimicrobial-enhancing agents.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Alkaloids, antimicrobial, bioautography, Glycosmis pentaphylla, photo-activated enzymatic restriction inhibition
Subjects:	R Medicine > RS Pharmacy and materia medica > RS403 Materia Medica-Pharmaceutical Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Basic Medical Sciences Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry Kulliyyah of Pharmacy
Depositing User:	Dr. Norazian Mohd Hassan
Date Deposited:	08 Nov 2022 08:45
Last Modified:	08 Nov 2022 08:56
URI:	http://irep.iium.edu.my/id/eprint/101028

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