Assessment of antibacterial activity of fresh garlic juice extract against Vibrio spp. isolated from hybrid grouper (Epinephelus fuscoguttatus x Epinephelus lanceolatus): An in vitro study

Razali, Muhamad Izzuan and Nik Yusoff, Nik Haiha and Mohd Idris, Shaharah and Abdullah, Azila and Ramly, Rimatulhana and Mohammad Ridzuan, Mohd Syafiq and Mansor, Nur Nazifah and Mustafa, Sufian and Nawi, Mohd Firdaus (2022) Assessment of antibacterial activity of fresh garlic juice extract against Vibrio spp. isolated from hybrid grouper (Epinephelus fuscoguttatus x Epinephelus lanceolatus): An in vitro study. In: 11th Symposium on Diseases in Asian Aquaculture (DAA11), 23-26 August 2022, Kuching, Sarawak. (Unpublished)

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Abstract

We assessed the antibacterial activity of fresh garlic juice extract against Vibrio spp. isolated from hybrid grouper (Epinephelus fuscoguttatus x Epinephelus lanceolatus). The disc diffusion method was conducted to evaluate five concentrations of fresh garlic juice extract against Vibrio alginolyticus, Vibrio vulnificus, and Vibrio parahaemolyticus. The tested concentrations are 20%, 40%, 60%, 80%, 100%, and oxytetracycline (OTC) antibiotic as a positive control, while absolute ethanol as a negative control. Results from the disc diffusion study revealed 100% and 80% garlic juice extract have higher inhibition zone against V. alginolyticus and V. vulnificus compared to OTC. The inhibition zone against V. parahaemolyticus was slightly less when 100% garlic juice extract gave an equal inhibition zone with OTC and smaller in other concentrations. Minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) were also studied, which resulted in the lowest MIC value was V.alginolyticus (0.011%) followed by V.vulnificus (0.023%) and the highest is V. parahemolyticus (0.045%). Similarly, the lowest MBC value was V. alginolyticus (0.023%) followed by V. vulnificus (0.045%) and the highest is V.parahemolyticus (0.09%). The results revealed that fresh garlic juice extract was effective against the test bacteria and showed the potential of using fresh garlic juice extract treatment as an antibacterial agent to treat vibriosis in hybrid grouper fish.

Item Type:	Proceeding Paper (Poster)
Uncontrolled Keywords:	Garlic, Hybrid Grouper, Vibrio spp., Antimicrobial activity
Subjects:	Q Science > QR Microbiology S Agriculture > SH Aquaculture. Fisheries. Angling S Agriculture > SH Aquaculture. Fisheries. Angling > SH151 Aquaculture - Fish Culture
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Marine Science
Depositing User:	Dr Mohd Firdaus Nawi
Date Deposited:	31 Oct 2022 09:26
Last Modified:	31 Oct 2022 09:26
URI:	http://irep.iium.edu.my/id/eprint/100857

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