Molecular phylogeny of holothuria (Mertensiothuria) leucospilota (Brandt 1835) as inferred from cytochrome C oxidase I mitochondrial DNA gene sequences

Kamarudin, Kamarul Rahim and Mohamed Rehan, Aisyah and Hashim, Ridzwan and Usup, Gires and Ahmad, Hajar Fauzan and Anua, Mohd. Hanafi and Idris, Mohd Yaman (2011) Molecular phylogeny of holothuria (Mertensiothuria) leucospilota (Brandt 1835) as inferred from cytochrome C oxidase I mitochondrial DNA gene sequences. Sains Malaysiana, 40 (2). pp. 125-133. ISSN 0126-6039

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Abstract

Holothuria (Mertensiothuria) leucospilota (Brandt 1835), white threads fish or locally known as bat puntil is currently considered as the most abundant sea cucumber species in Malaysia. This study aimed to generate the genetic profile of H. leucospilota from Malaysia and then to determine the phylogenetic relationship between H. leucospilota and other members of genus Holothuria using partial sequences of cytochrome c oxidase I (COI) mitochondrial DNA (mtDNA) gene. In this study, specimens of H. leucospilota were collected from Intan Besar Island, Langkawi, Kedah Darul Aman in the west coast of Peninsular Malaysia. Three main methods namely neighbour joining, maximum parsimony and maximum likelihood were used for the phylogenetic tree reconstruction. Tree topologies showed that H. leucospilota has its own monophyletic clade clearly distinct from the other species. The pairwise genetic distance calculated further supported these findings. In addition, the results also should that the COI mtDNA gene is capable to unravel the phylogenetic relationship of H. leucospilota. Keywords: Holothuria leucospilota; molecular systematics; partial sequences of cytochrome oxidase I gene; phylogeny tree

Item Type:	Article (Journal)
Additional Information:	4469/9905
Uncontrolled Keywords:	Holothuria leucospilota; molecular systematics; partial sequences of cytochrome oxidase I gene; phylogeny tree
Subjects:	Q Science > Q Science (General) R Medicine > RB Pathology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Science > Department of Biomedical Science
Depositing User:	Ms Rosyidah Taju Rahim
Date Deposited:	13 Dec 2011 16:59
Last Modified:	20 Dec 2011 08:01
URI:	http://irep.iium.edu.my/id/eprint/9905

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