Chemometrics-based evaluation on the effect of sonication, contact time and solid-to-solvent ratio on total phenolics and flavonoids, free fatty acids and antibacterial potency of Carica papaya seed against S. enteritidis, B. cereus, V. vulnificus and P. mirabilis

Abdullah Sani, Muhamad Shirwan and Bakar, Jamilah and Azid, Azman and Iqbal, Muhammad Javid (2022) Chemometrics-based evaluation on the effect of sonication, contact time and solid-to-solvent ratio on total phenolics and flavonoids, free fatty acids and antibacterial potency of Carica papaya seed against S. enteritidis, B. cereus, V. vulnificus and P. mirabilis. Food Chemistry Advances, 1 (100033). ISSN 2772-753X (In Press)

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Abstract

This study was aimed at extraction optimization of antibacterial agents from Carica papaya seed against S. enter-itidis, B. cereus, V. vulnificus and P. mirabilis as affected by sonication-assisted extraction (SAE), contact time (CT) and solid-to-solvent ratio (SSR). The principal component analysis (PCA) and individual evaluation approaches identified that no SAE, 8 CT and 1:10 SSR were the best treatments with the highest antibacterial potency. The PCA identified no SAE, 8 CT, and 1:5 SSR as the second-beat treatment. The yield, total phenolic compound (TPC), C18:1n9t and C16:1 free fatty acids (FAs) in no SAE, 8 CT and 1:10 SSR treatment inhibited B. cereus, V. vulnificus and P. mirabilis growths while C21:0 and C15:0 in 30 min SAE, 8 CT and 1:2 SSR inhibited S. enteritidis growth. The yield, TPC, C18:1n9t and C16:1 FAs, and C6:0 and C24:1n9, C20:1, C4:0 and C20:0 FAs had antagonistic effects on B. cereus, V. vulnificus and P. mirabilis growths. The C21:0, C15:0, C6:0 and C13:0, and C23:0, C20:0 and C11:0 FAs had antagonistic effects on S. enteritidis growth. The PCA also denoted that the MIC50 and MIC0 had a higher variation than MIC; hence, the former variables were better to use in PCA.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Sonication-assisted extraction Contact time Solid-to-solvent ratio Antibacterial potency Carica papaya seed
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART)
Depositing User:	Muhamad Shirwan Abdullah Sani
Date Deposited:	28 Apr 2022 09:09
Last Modified:	28 Apr 2022 09:09
URI:	http://irep.iium.edu.my/id/eprint/97720

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