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Optimum Medium Components for Biosurfactant Production by Klebsiella pneumoniae WMF02 Utilizing Sludge Palm Oil as a Substrate

Jamal, Parveen and Wan Nawawi, Wan Mohd Fazli and Alam, Md. Zahangir (2011) Optimum Medium Components for Biosurfactant Production by Klebsiella pneumoniae WMF02 Utilizing Sludge Palm Oil as a Substrate. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur.

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Abstract

In the present study, optimizing critical nutritional constituents was attempted as a primary strategy to improve biosurfactant production from Klebsiella pneumoniae WMF02 in liquid state fermentation utilizing sludge palm oil as a substrate. One-factor-at-a-time (OFAT) optimization was employed to evaluate the effects of sludge palm oil (SPO), sucrose, MgSO4, FeSO4, NaNO3 and K2HPO4 on surface tension reductivity. Sucrose was selected as a co-substrate over glucose in production media. The optimal levels of the aforementioned variables were (g/l) sucrose 5.0, MgSO4 0.4, FeSO4 0.3, NaNO3 2.0, and K2HPO4 4.0, with SPO concentration of 4% (v/v). The optimized medium shows surface tension reduction from 36.2 mN/m (non-optimized medium) to 25.70 mN/m. Preliminary biosurfactant identification indicated that the biosurfactant produced was phospholipids in nature.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 2937/9758 ISBN: 978-983-42978-3-1
Uncontrolled Keywords: component; biosurfactant; sludge palm oil, onefactor-at-a-time optimization (OFAT); klebsiella pneumoniae
Subjects: T Technology > TA Engineering (General). Civil engineering (General) > TA164 Bioengineering
T Technology > TA Engineering (General). Civil engineering (General) > TA170 Environmental engineering
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Prof.Dr. Parveen Jamal
Date Deposited: 14 Dec 2011 11:21
Last Modified: 14 Dec 2011 11:21
URI: http://irep.iium.edu.my/id/eprint/9758

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