The effect of maturity and extraction solvents on bioactive compounds and antioxidant activity of mulberry (Morus alba) fruits and leaves

Chen, Centhyea and Mohd Mokhtar, Ruzaidi Azli and Abdullah Sani, Muhamad Shirwan and Mohd Noor, Nor Qhairul Izzreen (2022) The effect of maturity and extraction solvents on bioactive compounds and antioxidant activity of mulberry (Morus alba) fruits and leaves. Molecules, 27 (8). E-ISSN 1420-3049

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Abstract

Cultivation location, maturity levels, and extraction solvents could affect the bioactive compounds and biological activities of mulberry (Morus alba Linnaeus). The lack of study on Malaysiagrown mulberry causes its underutilization. This study investigated the bioactive compound content and the antioxidant activity of Sabah-grown mulberry at two different maturity stages (fruits: red mature and black fully ripe; leaves: young and mature) extracted using 70% (v/v) methanol, 60% (v/v) ethanol, and 65% (v/v) acetone. Analyses showed that mulberry fruits demonstrated maturitydependent increment (except UHPLC-DAD quantification), while the leaves revealed maturitydependent reduction. Principal component analysis (PCA) displayed 65% (v/v) acetone black fully ripe fruits as the best phenolics and antioxidant sources. However, the 60% (v/v) ethanol black fully ripe fruits contained 20.08–68.43% higher total anthocyanins. Meanwhile, the 65% (v/v) acetone and 70% (v/v) methanol red mature fruits were higher in chlorogenic acid (27.53–47.12%) and rutin (31.42–35.92%) than other fruit extracts, respectively. For leaves, 65% (v/v) acetone young leaves were the best phenolics and antioxidant sources. However, the 60% (v/v) ethanol young leaves possessed greater chlorogenic acid (19.56–74.11%) than other leaf extracts. Overall, Malaysia-grown mulberry is rich in phenolics and antioxidants, suggesting its potential application in food and pharmaceutical products.

Item Type:	Article (Journal)
Uncontrolled Keywords:	bioactive compounds; antioxidant activity; UHPLC-DAD; chlorogenic acid; rutin; Morus alba Linnaeus; mulberry fruits; mulberry leaves; principal component analysis (PCA)
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART)
Depositing User:	Muhamad Shirwan Abdullah Sani
Date Deposited:	11 Apr 2022 09:07
Last Modified:	11 Apr 2022 09:07
URI:	http://irep.iium.edu.my/id/eprint/97561

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