Extraction of bioactive compound from Acacia seyal gum, in vitro evaluation of antitumor activity of its crude extract against leukemia

Kabbashi, Nassereldeen Ahmed and Alwadat, Tahani and Qudsieh, Isam Y. and Alam, Md. Zahangir and Shahabuddin, Munirah (2022) Extraction of bioactive compound from Acacia seyal gum, in vitro evaluation of antitumor activity of its crude extract against leukemia. International Journal of Plant Based Pharmaceuticals, 2 (1). pp. 82-88. E-ISSN 2791-7509

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Abstract

Today, many therapy drugs have been used to treat cancer patients. However, those drugs are not effective enough and usually have adverse side effects on human health. Different herbal medicine is rising in popularity because it is more compatible with the human body and has fewer side effects. Even while alternative herbal remedies effectively decrease symptoms in traditional medicine, many of them have yet to be scientifically proven. As a result, it's critical to keep looking for ways to recover its efficiency against cancer cells. Acacia seyal gum (ASG), known as Arabic gum, is a well-known traditional medicinal therapy with various restorative characteristics. In this study, the yield of ASG extract was optimized using experimental design followed by chemical characterization of a bioactive compound for the last yield, then the therapeutic potential of ASG crude extracts against leukemia cancer cells was investigated in vitro. The Raman Spectroscopy (RS), Fourier Transform Infrared (FTIR) Spectroscopy, and GC-TOFMS analyses were used to characterize ASG crude hydroethanolic extract bioactive components. The anti-leukemic activity of ASG crude extracts was investigated in vitro against tumoral Jurkat T-cell ALL, and K562 leukemia cancer cell lines, as well as nontumoral WIL2NS cells. The optimum extraction conditions resulted in a yield of 75.89% after 45 min of extraction at temperature 40 °C and solid/liquid ratio of 1:25 g/ml. The cytotoxicity assays of ASG and Taxol revealed that both treatments inhibited the growth of K562 and Jurkat T cancer cells and exhibited the lowest IC50 for K562 and Jurkat T cancer cell (IC50=10 g/ml and IC50=5.11 g/ml, respectively), and a negligible inhibition effect for WIL2NS cells (IC50=80 g/ml).

Item Type:	Article (Journal)
Additional Information:	4286/96467
Uncontrolled Keywords:	Acacia seyal gum; Sonication; FTIR spectroscopy; Raman spectroscopy; Anticancer
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering Kulliyyah of Engineering
Depositing User:	Dr Nassereldeen Kabbashi
Date Deposited:	25 Jan 2022 12:14
Last Modified:	25 Jan 2022 12:14
URI:	http://irep.iium.edu.my/id/eprint/96467

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