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Process scale-up criteria in production of recombinant proteins in E. coli: a systematic review

Mohamed Gameil, Abeir Hussein and Yusof, Faridah and Azmi, Azlin Suhaida and Mohamad Puad, Noor Illi (2021) Process scale-up criteria in production of recombinant proteins in E. coli: a systematic review. Biological And Natural Resources Engineering Journal, 5 (2). pp. 37-61. E-ISSN 2637-0719 (In Press)

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This systematic review provides an overview of the scale-up of recombinant protein production. A systematic search was conducted using pre-established terms in four electronic databases (PubMed, Europe PMC, Science Direct, and Scopus), and included journal articles published between 1999 to 14 November 2019. Studies that met the inclusion and exclusion criteria specified were included. Initially, 665 abstracts were screened, of which 595 were excluded. A further 58 articles were excluded after full-text review and14 articles underwent quality assessment. Finally, only eight (8) eligible articles were identified and included in this review. Oxygen transfer, shear, and production costs were identified as the main factor that affected the choice and success of a scale-up strategy. The comparison of different fermentation systems revealed that maintaining the kLa constant was found to be effective in all included studies which employed it, and superior in the two studies that compared it to other criteria. Risk of bias assessment revealed that publication bias and reporting bias are significant issues to be considered in scale-up. Overall, these findings provide insight that can assist researchers to improve the scaling up of fermentation processes and achieve a successful scale-up of recombinant protein production using E. coli as the host.

Item Type: Article (Journal)
Additional Information: 5462/95252
Uncontrolled Keywords: Scale-up, Escherichia coli, Fermentation, Recombinant Protein, Bioreactor
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering
Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Noor Illi Mohamad Puad
Date Deposited: 31 Dec 2021 11:45
Last Modified: 31 Dec 2021 11:46
URI: http://irep.iium.edu.my/id/eprint/95252

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