Optimisation of vitamin B12 extraction from green edible seaweed, Ulva lactuca using Central Composite Design

Darnis, Deny Susanti (2021) Optimisation of vitamin B12 extraction from green edible seaweed, Ulva lactuca using Central Composite Design. In: 3rd International Seminar on Science and Technology, 30th November 2021, Virtual Conference. (Unpublished)

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Abstract

Vitamin B12, which is essential for brain function and a variety of chemical reactions in the body, can be found naturally only in animal foods. Insufficient intake of vitamin B12 lead to vitamin B12 deficiency which is common among strict vegetarians due to their limited intake of animal-based foods. However, extensive studies showed that macroalgae species, specifically Ulva lactuca are rich in vitamin B12 content and can also be further exploited for its dietary applications in the future. In this study, the best extraction method of vitamin B12 from dried U. lactuca was being developed and optimised to achieve the maximum yield of vitamin B12. Also, the effect of several extraction parameters namely; solvent:solvent (MeOH:H2O), solute:solvent ratio, and pH on the total vitamin B12 content were analysed individually by 2-Level Factorial and Central Composite Design. Results showed that highest vitamin B12, particularly Cyanocobalamin (CN-Cbl) content can be recovered through the ultrasonic-assisted extraction (UAE) of oven-dried U. lactuca, at 3 g: 60 mL solute: solvent, 25: 75 % MeOH: H2O ratios and at pH 4. The extraction of CN-Cbl from oven-dried U. lactuca using UAE method has improved the CN-Cbl content as compared with other types of dried U. lactuca using different extraction methods.

Item Type:	Conference or Workshop Item (Invited Papers)
Additional Information:	5641/94548
Uncontrolled Keywords:	Extraction, vitamin B12, green seaweed, Ulva lactic
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Chemistry
Depositing User:	Dr Deny Susanti Darnis
Date Deposited:	29 Dec 2021 14:15
Last Modified:	29 Dec 2021 16:56
URI:	http://irep.iium.edu.my/id/eprint/94548

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