Angiotensin-converting enzyme inhibition activity and LCMS analysis of Syzygium polyanthum (Wight) Walp leaves

Tuan Anuar, Tuan Ashraf Faiz and Ismail, Azlini and Omar, Muhammad Nor and Mohamed Suffian, Izzat Fahimuddin and Abdul Hamid, Azzmer Azzar (2021) Angiotensin-converting enzyme inhibition activity and LCMS analysis of Syzygium polyanthum (Wight) Walp leaves. In: National Symposium on Essential Oils, Plants, Cosmetic and Health, School of Health Sciences, Universiti Sains Malaysia.

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Abstract

Syzygium polyanthum is an ethnomedicinal plant that is widely used among the Malay community for treating hypertension. Previous studies have shown that the plant leaves have a significant antihypertensive effect in rats; however, its mechanism of action and the bioactive compounds is not yet well-studied. One of the possible mechanisms to reduce blood pressure is through inhibiting the angiotensin-converting enzyme (ACE). Therefore, this study aims to determine the ACE inhibitory activity of S. polyanthum leaves and its phytochemical composition. S. polyanthum leaves were dried, ground, macerated with water in a bath-sonicator, and then freeze-dried to produce the aqueous S. polyanthum (ASP) leaves extract. The ACE inhibition activity of ASP was tested using Cushman and Cheung method at a range of concentrations from 1 to 1000 µg/ml. The phytochemical composition in ASP was then analysed using Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (LCQTOF/MS). It was found that the concentration of ASP that causes 50 % of ACE inhibition activity (IC50) was 41 μg/ml. Through LC-QTOF/MS analysis, there were 26 compounds identified in ASP with the following major compounds; 1-galloyl-glucose (21%), feroxin A (12%), nilocitin (10 %), 2,6-di-O-galloyl-β-D-glucose (9%), 1-O-galloylpedun-culagin (7%), 5-desgalloylstachyurin (6 %), and yakuchinone A (6 %). In conclusion, this study showed that ASP which majorly consists of 1-galloyl glucose exhibited significant ACE inhibitory activity. Further bioassay-guided isolation is required to determine whether 1-galloyl glucose is the potential bioactive compound responsible for this activity.

Item Type:	Conference or Workshop Item (Other)
Uncontrolled Keywords:	Angiotensin converting enzyme, ACE, ACEi, hypertension, Syzygium polyanthum
Subjects:	Q Science > Q Science (General) R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology R Medicine > RM Therapeutics. Pharmacology > RM300 Drugs and their action
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry Kulliyyah of Science Kulliyyah of Science > Department of Biotechnology Kulliyyah of Science > Department of Chemistry
Depositing User:	DR. AZLINI ISMAIL
Date Deposited:	26 Oct 2021 16:02
Last Modified:	26 Oct 2021 16:02
URI:	http://irep.iium.edu.my/id/eprint/92974

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