Sperm morphology and testis histological changes in 12% high cholesterol diet administered rats following Tualang honey supplementation and diet modifications

Hasan, Sakiinah and Abdul Rahim, Roslina and Alias, Mohd Afzal and Muhammad, Siti Aeshah @ Naznin and Abdullah, Nor Zamzila and Abdul Razak, Redzuan Nul Hakim (2021) Sperm morphology and testis histological changes in 12% high cholesterol diet administered rats following Tualang honey supplementation and diet modifications. In: MSPP 34th Scientific Meeting 2021: Trailblazing The Translational Research, Monash University Malaysia (Virtual Meeting). (Unpublished)

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Abstract

Background: Hypercholesterolaemia is recognized as a factor associated with male infertility. However, currently, there is limited therapy available. Tualang honey (TH) is a type of Malaysian polyfloral wild honey produced by the rock bee (Apis dorsata) proven to exert both anti-inflammatory and anti-oxidative effects. Objective: This study aimed to determine the effects of TH coupled with diet modification on sperm morphology and testis histology of 12% high cholesterol diet (HCD) administered rats. Methodology: Twenty rats were divided into 4 groups. Control group were fed with standard diet for 20 weeks. The other 3 groups were fed with 12%HCD for 16 weeks. Then, group A rats were not treated and continued HCD. Group B rats were supplemented with TH (3.0g/kg) and continued HCD, while group C was given TH (3.0g/kg) but was changed to a normal diet for 4 weeks. At the end of 20 weeks, the left testis was collected for histology analysis and the cauda epididymis was collected to analyse the sperm parameter. Results: Compared to control, group A showed significant decrease in the percentage of normal sperm morphology (p<0.001). In contrast, group B and C demonstrated significant improvement in sperm morphology compared to group A (p<0.001). Group C showed better improvement in the percentage of normal sperm morphology compared to group B although not significant (p>0.05). However, there are no significant changes in Johnsen testicular histology scoring between the groups. Conclusion: Diet modification in addition to TH supplementation may further improve male fertility in HCD rats. Based on our findings, there is a need to further explore the potential TH in improving male infertility associated with hypercholesterolaemia.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	5456 / 91806
Uncontrolled Keywords:	High cholesterol diet, Tualang honey, sperm morphology, testis histology, Johnsen testicular scoring
Subjects:	Q Science > QP Physiology R Medicine > R Medicine (General) R Medicine > RB Pathology R Medicine > RC Internal medicine R Medicine > RC Internal medicine > RC627 Specialties of Internal Medicine-Metabolic Diseases R Medicine > RG Gynecology and obstetrics R Medicine > RZ Other systems of medicine
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine Kulliyyah of Medicine > Department of Basic Medical Kulliyyah of Medicine > Department of Pathology & Lab Medicine Kulliyyah of Nursing Kulliyyah of Nursing > Department of Basic Medical Sciences for Nursing
Depositing User:	Dr ROSLINA ABDUL RAHIM
Date Deposited:	23 Sep 2021 10:25
Last Modified:	06 Apr 2022 10:16
URI:	http://irep.iium.edu.my/id/eprint/91806

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