Identification of potential biomarkers of porcine gelatin

Tukiran, Nur Azira and Ismail, Amin and Ghazali, Haizatul Hadirah and Mohd Ali, Nurul Azarima (2021) Identification of potential biomarkers of porcine gelatin. In: Multifaceted protocols in biotechnology. Springer, Cham, Switzerland, pp. 93-101.

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Abstract

This chapter discusses the identification of potential biomarkers of porcine gelatin using peptide mass fingerprinting (PMF) for the development of anti-peptide polyclonal antibodies. The selection of antigens is a prerequisite for the development of enzyme-linked immunosorbent assay (ELISA). A synthetic peptide is one of the prevalent antigens for ELISA development. It enables the produced antibodies to be targeted at small regions of the protein with fine specificity. It has been used to solve food authenticity issues.

Item Type:	Book Chapter
Additional Information:	7613/91619
Uncontrolled Keywords:	SDS-PAGE, Peptide mass fingerprinting, In-gel tryptic digestion, Polypeptide band, Porcine gelatin
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART)
Depositing User:	Mrs. Wan Salwati Wan Salleh
Date Deposited:	20 Aug 2021 11:36
Last Modified:	23 Aug 2021 09:24
URI:	http://irep.iium.edu.my/id/eprint/91619

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