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Progressive hepatic histopathological changes in high cholesterol diet induced non-alcoholic fatty liver disease model

Ahmed Mohamed Ben-Amer, Nawal and Buyong, Zunariah and Abdullah, Nor Zamzila and Hamdan, Asmah Hanim and Ab. Rahman, Jamalluddin and Kuttulebbai1, Sirajudeen (2021) Progressive hepatic histopathological changes in high cholesterol diet induced non-alcoholic fatty liver disease model. International journal of allied health sciences, 5 (1). p. 2181. E-ISSN 2600-8491

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Abstract

Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease. The disease ranging from simple hepatic steatosis to progressive steatohepatitis (NASH). Recently model of NASH was established following 12% cholesterol diet for six weeks. However, histopathological changes before developing NASH were not yet determined. This study aimed to ascertain NAFLD progression at four, five and six weeks. Twelve Sprague Dawley rats were divided into four groups. Control received normal diet, and another three were fed on 12% cholesterol diet for four, five and six weeks. Body and liver weight were measured. Liver tissues stained for haematoxylin and eosin staining. The percentage of steatosis was measured using Image J software. The differences between groups were analysed using One-way ANOVA. There were significant increase in body weight in five and six week groups compared to control, while liver weight was significantly raised in six weeks. Progressive hepatic changes were observed from four to six weeks. Four weeks showed simple steatosis, whereas ballooning degeneration and inflammation were more obvious in six than five weeks. In conclusion, this model demonstrated the histopathological progression of NAFLD.

Item Type: Article (Meeting Abstract)
Uncontrolled Keywords: Keywords: Non-alcoholic fatty liver disease.
Subjects: R Medicine > RB Pathology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Medicine > Department of Basic Medical
Kulliyyah of Medicine > Department of Community Medicine (Effective: 1st January 2011)
Kulliyyah of Medicine > Department of Pathology & Lab Medicine
Depositing User: DR ASMAH HANIM HAMDAN
Date Deposited: 05 Jul 2021 16:19
Last Modified: 05 Jul 2021 16:19
URI: http://irep.iium.edu.my/id/eprint/90520

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