Ascorbic acid loaded PLGA nanoparticles gel intended to treat oral squamous cell carcinoma

Mohammad Hamdi, Nurul Ain and Haris @ Harith, Muhammad Salahuddin and Harun Ismail, Ahmad Fahmi (2021) Ascorbic acid loaded PLGA nanoparticles gel intended to treat oral squamous cell carcinoma. In: 6th International Conference on Biotechnology Engineering 2021, Kuala Lumpur, Malaysia (Virtual). (Unpublished)

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Abstract

The conventional chemotherapy approach is associated with several drawbacks specifically detrimental adverse effects to the patient and occurrence of chemoresistance towards commonly used chemotherapy which further leads to treatment failure, disease recurrence and metastasis. The main objective of this study was to develop and characterise alternative potential anticancer ascorbic acid loaded PLGA nanoparticles gel for oral mucosa application for the treatment of oral squamous cell carcinoma. Ascorbic acid loaded PLGA nanoparticles were prepared by double emulsion solvent evaporation method followed by incorporation in different concentration of mucoadhesive Carbopol gel bases. The nanoparticles formulation were characterised for various physicochemical properties such as particle size, polydispersity index, zeta potential, encapsulation efficiency, drug-polymer interaction, nanoparticles morphology and in vitro drug release profile. Ascorbic acid nanoparticle loaded mucoadhesive gel were evaluated for physical appearance, pH, viscosity, flow behaviour, spreadability, mucoadhesion and in vitro release study. The particle size of the optimised nanoparticles was found to be 252 ± 2.98 nm, polydispersity index of 0.151 ± 0.02, zeta potential of -20.93 ± 0.87 mV and encapsulation efficiency of 69.73 ± 1.07%. Scanning electron microscope images revealed the spherical shape of nanoparticles. The drug release behaviour exhibited a biphasic pattern namely initial burst release followed by controlled release subsequently. The FT-IR result confirmed the absence of drug-polymer interaction. The optimised nanoparticle-in-gel formulation showed a good physical appearance, pH value, spreadability, viscosity and mucoadhesion. The flow behaviour of the optimised nanoparticle-in-gel formulation exhibited pseudoplastic behaviour. The cumulative amount of ascorbic acid released at 6 hours was 42.9 ± 4.3% with zero-order release kinetics. In conclusion, ascorbic acid loaded PLGA nanoparticle-in-mucoadhesive gel was successfully prepared and the study proved the potentiality and suitability of the formulation to be topically applied to treat oral squamous cell carcinoma.

Item Type:	Conference or Workshop Item (Speech/Talk)
Additional Information:	6657/90492
Uncontrolled Keywords:	Ascorbic acid, PLGA, nanoparticles, mucoadhesive gel, oral cancer treatment
Subjects:	R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Physical Rehabilitation Sciences Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User:	Dr. Muhammad Salahuddin Haris
Date Deposited:	08 Jul 2021 17:18
Last Modified:	08 Jul 2021 17:18
URI:	http://irep.iium.edu.my/id/eprint/90492

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