Ruslan, Fatin Shazwani and Darnis, Deny Susanti and Mohammad Noor, Normawaty and Bakhtiar, M. Taher and Aminudin, Nurul Iman
(2021)
Screening of factors affecting the vitamin B12 extraction from Ulva Lactuca by 2-level factorial design.
In: YSN-ASM International Scientific Virtual Conference (ISVC) 2021, 29th March - 1st April 2021, Virtual.
(Unpublished)
Abstract
Abstract
Introduction: Ulva lactuca possess many biological properties due to the presence of bioactive compounds. It is believed to be an alternative source for vitamin B12 for strict vegetarians. Methods: The extraction of vitamin B12 was performed using boiling extraction method from freeze-dried (FDB), air-dried (ADB), oven-dried (ODB) and sun-dried (SDB) sample. The effect of solvent:solvent, solute:solvent ratios and pH on total vitamin B12 content extracted from the different dried samples were investigated and analysed using 2-Level Factorial design from RSM. The vitamin B12 (methylcobalamin, hydroxocobalamin, adenosylcobalamin and cyanocobalamin) were analysed quantitatively and qualitatively using HPLC. Results: The retention time (RT) of all samples corresponded to the RT of standard cyanocobalamin (CNCbl), at 1.9 min. ODB sample yielded the highest concentration of CNCbl (0.0210 mg/mL) when extracted at 25:75 % methanol:water, 3 g:60 mL solute:solvent and at pH 3, respectively. The overall model and interaction effect between solvent:solvent ratios and pH, as well as between solvent:solvent and solute:solvent ratios were significant for ODB sample at p-value less than 0.05, scrutinised by ANOVA analysis. Conclusion: Different methods of drying, solvent:solvent, pH and solute:solvent have different effect on the CNCbl content extracted from U. lactuca.
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