Mazlan, Muhammad Alif and Md Isa, Muhammad Lokman and Ibrahim, Moustafa
(2020)
A high mannose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: an in vitro investigation.
Egyptian Journal of Medical Human Genetics, 21 (1).
ISSN 11108630
Abstract
Background: The primary cause of cancer is gene mutation which allows the growth of abnormal and damaged
cells. Nutrition is one of the key factors that either increases or decreases the risk of cancer. Mannose has been
found in many fruits such as oranges, apples and berries. Mannose has been linked to increase the risk factors or
potential therapeutic for cancers. However, insufficient information is available on the effects of high mannose
concentration on the normal and cancer cell lines. This study aimed to evaluate the viability patterns of human
cancer and normal cell lines treated with mannose. Human gingival fibroblast (HGF), skin malignant melanoma
(A375) and colorectal adenocarcinoma (HT29) cell lines were cultured and treated with additional mannose in three
respective concentrations: 1 mg/ml, 5 mg/ml and 10 mg/ml. Then, cell viability was measured using an MTT (3-(4,5-
dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide)-assay.
Results: The HGF cells’ percentage pattern of viability showed a rapid decline of nearly 95% on the third day of
treatment. A375 cells were able to survive in high mannose condition as the cell viability percentage was at the
highest value on Day 5. Meanwhile, HT29 cells showed declining cell viability pattern when treated with mannose.
The data exhibited significance; the p value was less than 0.001.
Conclusions: High mannose concentration can be toxic to HGF. In addition, A375 is adaptive to mannose at all
concentrations in which it shares the same pattern with the untreated group. However, the cell viability pattern for
HT29 cell is declining
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