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The thrombolytic and cytotoxic effects of nigella sativa (L.) seeds: the prophetic medicine

Babar, Z.M. and Jaswir, Irwandi and Maifiah, Mohd Hafidz Mahamad and Ismail, Soraya and Raus, Raha Bt. Ahmad and Tareq, A. M. and Ahfter, F. and Faraque, A. and Ali Reza, A.S.M and Sayeed, M. A. and Hossain, Md. M. and Uddin, M. and Hossain, M. S. and Farhad, S. and Azizi, W.M. and Arief Ichwan, Solachuddin Jauhari and Ahmed, Qamar Uddin and Mawa, I. (2020) The thrombolytic and cytotoxic effects of nigella sativa (L.) seeds: the prophetic medicine. International Journal of Halal Research, 2 (2). pp. 70-77. ISSN 2721-7868

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The Water-Soluble Extract (WSE) is a crude bioactive phytoconstituent of Nigella sativa (L.) seeds discovered recently. The current findings report about the thrombolytic and cytotoxic effects of WSE using human blood clot lysis and brine shrimp lethality (BSL) bioassay. The thrombolytic effect of WSE (1,666.67 µg/mL) was determined via the clot and lysate weight measurements compared to streptokinase (STK) of 30,000 IU/mL and normal saline (NS) while the cytotoxicity of WSE (44.14-2,000 µg/mL) against vincristine sulfate (VCS;3.125-100 µg/mL). WSE has shown extremely statistically significant (p<0.0001) clot lysis (90.00%) compared to NS (3.76%) whilst it was also significantly different (p<0.0063) to STK (72.41%) exhibiting LC50 of 1,795.90 µg/mL vs. VCS (39.25 µg/mL) in a dose-dependent manner. The current results suggested WSE has a potent thrombolytic effect with mild dose-dependent cytotoxicity towards brine shrimp nauplii (Artemia salina). It also suggested WSE might have enzymatic roles on thrombin, fibrin, and plasmin of blood. This pharmacological action of WSE is might be due to its antioxidant property, short-chain fatty acids and/or amino acids. Further studies are highly recommended on the enzymatic role(s) and bioactive phytoconstituents of WSE.

Item Type: Article (Journal)
Uncontrolled Keywords: Brine Shrimp Nauplii (Artemia salina) Cytotoxicity Human Blood Clot Thrombolysis Water-Soluble Extract (WSE)
Subjects: Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): International Institute for Halal Research and Training (INHART)
Kulliyyah of Dentistry
Kulliyyah of Engineering
Kulliyyah of Engineering > Department of Biotechnology Engineering
Kulliyyah of Medicine
Kulliyyah of Medicine > Department of Basic Medical
Kulliyyah of Pharmacy
Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User: Prof. Dr. Irwandi Jaswir
Date Deposited: 05 Jan 2021 12:19
Last Modified: 05 Jan 2021 12:19
URI: http://irep.iium.edu.my/id/eprint/87057

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