Determination of gossypol in Hamid and Bt (Seeni 1) cottonseed oil using fourier transform infrared spectroscopy

Abdelrahman, Samah A. M. and Yassin, Atif A. A. and Mirghani, Mohamed Elwathig Saeed and Bashir, Nabil H. H. (2020) Determination of gossypol in Hamid and Bt (Seeni 1) cottonseed oil using fourier transform infrared spectroscopy. Borneo Journal of Pharmacy, 3 (4). 227 - 234. E-ISSN 2621-4814

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Abstract

This study was conducted to determine the gossypol content in Bt cottonseed (Seeni-1) oil by using Fourier Transform Infrared (FTIR) spectroscopy with an Attenuated Total Reflectance (ATR) element. The wavelengths used were selected by spiking refined, bleached deodorized palm oil (RBDPO) to gossypol concentrations of 0-5% and noting the regions of maximal absorbance. Absorbance values of the wavelength regions 3700-2400 & 1900-750 cm−1 and a partial least squares (PLS) method were used to derive calibration models for Hamid cottonseed oil, Seeni-1 cottonseed oil, and gossypol-spiked RBDPO. The coefficients of determination (R2) for the calibration models were computed for the FTIR spectroscopy results against those found by using the wet chemical method AOCS method Ba 8–78. The R2 was 0.8916, 0.9581, and 0.9374 for Hamid cottonseed oil, Seeni-1 cottonseed oil, and gossypol-spiked RBDPO, respectively. The standard error (SE) of the calibration was 0.053, 0.078, and 0.062, respectively. The calibration models were validated using the cross-validation technique within the same set of oil samples. The results of FTIR spectroscopy as a useful technique determining gossypol content in crude cottonseed oil showed that there is a significant difference (p<0.05) in the amount of gossypol content in Hamid and Bt Seeni-1 cottonseed oils.

Item Type:	Article (Journal)
Additional Information:	4971/85652
Uncontrolled Keywords:	Bacillus thuringiensis, Cottonseed oil, FTIR spectroscopy Gossypol PLS
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART)
Depositing User:	A Prof Elwathig Mirghani
Date Deposited:	04 Dec 2020 15:29
Last Modified:	04 Dec 2020 15:29
URI:	http://irep.iium.edu.my/id/eprint/85652

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