Effect of trihoney (a mixture of trigona, mellifera and tualang) on male reproductive hormones and insulin resistance in hypercholesterolaemic rabbits

Hamad Mohamed, Zenab and Ibrahim, Muhammad and Hamad Alfarisi, Hamad Abdulsalam and Abdul Wahab, Azantee Yazmie and Abd Fuaat, A and Che Mohamad, Che Anuar (2020) Effect of trihoney (a mixture of trigona, mellifera and tualang) on male reproductive hormones and insulin resistance in hypercholesterolaemic rabbits. International Medical Journal Malaysia, 19 (3). pp. 21-29. E-ISSN 1823-4631

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Abstract

Introduction: Hypercholesterolaemia affects Sertoli and Leydig cells secretory functions, impairs steroid hormone biosynthesis, and disrupts the hypothalamic-pituitary-gonadal axis. The use of honey in previous studies resulted in an improvement of male reproductive hormonal disturbances. This study aimed to investigate the protective effects of Trihoney on hypercholesterolemia-induced male reproductive hormonal changes in male rabbits and compare its effects with atorvastatin. Materials and methods: Forty-eight male New Zealand white rabbits were assigned into 6 groups as follows; Control: commercial pellet; CH: commercial pellet with 0.6 g/kg/day Trihoney; HCD: 1% cholesterol diet; DH1: 1% cholesterol diet with 0.3 g/kg/day Trihoney; DH2: 1% cholesterol diet with 0.6 g/kg/day Trihoney and DAt: 1% cholesterol diet with 2mg/kg/day atorvastatin. After 12 weeks, the rabbits were sacrificed and blood samples were collected for analysis of hormones and pro-inflammatory cytokines and calculation of HOMA-IR. The testes were homogenized for intra-testicular testosterone measurement. Results: Serum testosterone reduced significantly in HCD (p<0.05) and DAt (p<0.05) groups. Likewise, intra-testicular testosterone reduced significantly in HCD (p<0.01) and DAt (p<0.01) groups. Serum FSH increased significantly in HCD (p<0.001) and DAt (p<0.01). Trihoney particularly at the dose of 0.6 g/kg/day improved serum and intra-testicular testosterone (p<0.05) and FSH (p<0.05). Trihoney and atorvastatin improved serum pro-inflammatory cytokines. Trihoney and atorvastatin did not affect HOMA-IR. Conclusion: Trihoney attenuated the detrimental effects of hypercholesterolaemia on male reproductive hormones which probably through a local effect on testicular tissue and Trihoney anti-inflammatory effect independent of insulin resistance. Atorvastatin did not counteract the impact of hypercholesterolaemia on the reproductive hormones.

Item Type:	Article (Journal)
Additional Information:	4669/85301
Uncontrolled Keywords:	Hypercholesterolaemia; Trihoney; Atorvastatin; Male reproductive hormones ; Insulin resistance
Subjects:	R Medicine > RB Pathology R Medicine > RG Gynecology and obstetrics R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Nutrition Sciences Kulliyyah of Medicine > Department of Obstetric & Gynecology Kulliyyah of Medicine > Department of Pathology & Lab Medicine Kulliyyah of Pharmacy > Department of Basic Medical Sciences
Depositing User:	salina mohammad
Date Deposited:	26 Nov 2020 14:46
Last Modified:	21 Nov 2023 09:21
URI:	http://irep.iium.edu.my/id/eprint/85301

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