Effects of cannabinoid CB1 receptor activation upon exercise-induced insulin stimulated glucose uptake in human primary skeletal muscle myotubes

Mohd Noor, Mohd Fadly and Bennett, Andrew J. and Tsintzas, Kostas (2016) Effects of cannabinoid CB1 receptor activation upon exercise-induced insulin stimulated glucose uptake in human primary skeletal muscle myotubes. In: University of Nottingham Postgraduate Research Symposium 2016, 14th - 15th July 2016, Nottingham. (Unpublished)

	PDF (Presentation Slides) - Presentation Restricted to Repository staff only Download (1MB) | Request a copy
	PDF (Event Booklet) - Supplemental Material Restricted to Repository staff only Download (1MB) | Request a copy

Abstract

Endocannabinoid system (ECS) plays an important role in the regulation of energy balance and metabolism. Studies showed that the activation of cannabinoid receptor type-1 (CB1) may lead to increase in food intake, weight gain, obesity and impairment in glucose tolerance. The aim of this study is to examine the effects of CB1 activation upon exercise-induced insulin stimulated glucose uptake in human primary skeletal muscle myotubes. We have developed a contractile myotubes model using electrical pulse stimulation (EPS) which demonstrates elevation in glucose transporter 4 (GLUT4), together with increased in insulin stimulated glucose uptake by the cells after EPS. Activation of CB1 by Arachidonoyl 2'-Chloroethylamide (ACEA) resulted in significant reduction in insulin stimulated glucose uptake and this effect were reverted with pre-treatment with CB1 antagonist: Rimonabant (RIM). Protein expression by western blotting revealed the activity of Akt/protein kinase B in insulin signalling pathway was inhibited by ACEA and this may suggest the mechanism of the inhibition of glucose uptake. In conclusion, activation of CB1 by ACEA inhibit exercise-induced insulin stimulated glucose uptake in human primary skeletal muscle myotubes via inhibition of Akt/protein kinase B activity and the effects was blunted by pre-treatment with RIM.

Item Type:	Conference or Workshop Item (Slide Presentation)
Additional Information:	6062/84623
Uncontrolled Keywords:	Cannabinoid, CB1 receptor, skeletal muscle myotubes, exercise, electrical pulse stimulation
Subjects:	Q Science > QP Physiology R Medicine > RM Therapeutics. Pharmacology R Medicine > RM Therapeutics. Pharmacology > RM300 Drugs and their action R Medicine > RM Therapeutics. Pharmacology > RM695 Physical medicine. Physical therapy
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine Kulliyyah of Medicine > Department of Basic Medical
Depositing User:	DR. MOHD FADLY MOHD NOOR
Date Deposited:	10 Nov 2020 11:02
Last Modified:	10 Nov 2020 11:02
URI:	http://irep.iium.edu.my/id/eprint/84623

Actions (login required)

View Item