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Antihypertensive assay-guided fractionation of Syzygium polyanthum leaves and phenolics profile analysis using LCQTOF/MS

Ismail, Azlini and Abd Rahim, Erlena Nor Asmira and Omar, Muhammad Nor and Wan Ahmad, Wan Amir Nizam (2020) Antihypertensive assay-guided fractionation of Syzygium polyanthum leaves and phenolics profile analysis using LCQTOF/MS. Pharmacognosy Journal, 12 (6(S)). pp. 1670-1692. ISSN 0975-3575

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Introduction: Syzygium polyanthum leaves extract that contains gallic acid as the major phenolic compound has shown significant antihypertensive effect, however the amount of gallic acid was inversely-related with magnitude of this effect. This study aimed to conduct bioassay-guided fractionation of S. polyanthum leaves with gallic acid as a reference compound, and to screen for other possible compounds responsible for the antihypertensive effect. Methods: S. polyanthum leaves were extracted using n-hexane, ethyl acetate, methanol, and water. The most active crude extract was fractionated using column chromatography and analyzed for total phenolic content (TPC) (n=3). Crude extracts and the derived fractions were intravenously administered into pentobarbital-anaesthetized Spontaneously Hypertensive rats (n=5) for recording of blood pressure parameters. Liquid Chromatography-Quadruple Time-Off-Flight/Mass Spectrometry was used for determination of chemical composition. One-way and two-way ANOVA were used for statistical analysis using GraphPad® PRISM Version 6. Results: Fractionation of aqueous S. polyanthum leaves extract (ASP) afforded nine fractions, later combined into three fractions (F1ASP, F2ASP, and F3ASP) based on the thin layer chromatography profiles. ASP has the highest TPC while F2ASP has the lowest TPC. All fractions exhibited significant antihypertensive property, but F2ASP was the most active fraction. Few phenolics with related antihypertensive effects such as 1-galloyl glucose (a gallic acid-derivative majorly found in F2ASP and F3ASP), and other compounds such as polydatin, sesamol, brazilin, eugenol, ellagic acid, kukoamine A, and cyclocurcumin were found across all active fractions. Conclusion: These phenolics may partly contribute to the antihypertensive effect of S. polyanthum leaves, thus further isolation study is recommended.

Item Type: Article (Journal)
Additional Information: 7407/84377
Uncontrolled Keywords: Antihypertensive, Bioassay-guided, LCMS, Syzygium polyanthum, Total phenol content (TPC)
Subjects: R Medicine > RM Therapeutics. Pharmacology
R Medicine > RM Therapeutics. Pharmacology > RM300 Drugs and their action
R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences
Kulliyyah of Dentistry
Depositing User: DR. AZLINI ISMAIL
Date Deposited: 06 Nov 2020 20:45
Last Modified: 09 Feb 2021 09:05
URI: http://irep.iium.edu.my/id/eprint/84377

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