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Gene expression analysis of fruit bromelain in ripening of Ananas comosus cultivar MD 2

Pang, Wei Cheng and Ramli, Aizi Nor Mazila and Abdul Hamid, Azzmer Azzar (2020) Gene expression analysis of fruit bromelain in ripening of Ananas comosus cultivar MD 2. In: Materials Science Forum. Trans Tech Publications Ltd, Kuantan, Pahang, pp. 209-214. ISBN 978-303571664-1

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Fruit bromelain is a proteolytic enzyme harbouring cysteine catalytic residue found abundantly in pineapple fruit. The expression of cysteine proteases is usually regulated during fruit ripening. In the present study, we aimed to study the expression and proteolytic activity level of fruit bromelain during the ripening stage of A. comosus cultivar MD 2. The gene expression of fruit bromelain was investigated via relative gene expression analysis using qPCR while the proteolytic activity of fruit bromelain was analysed via enzymatic assay using casein as a substrate. The qPCR analysis revealed that the expression of fruit bromelain was down-regulated 10-fold in ripe pineapple fruits. Besides that, the unripe pineapple fruits [1.9101 ± 0.0831 U/mL] had a higher proteolytic activity than the ripe MD 2 pineapple fruits [1.1333 ± 0.0896 U/mL]. This result showed that the function of fruit bromelain may be related to the protection of young pineapple fruits during the fruit development stage.

Item Type: Book Chapter
Additional Information: 7089/82591
Uncontrolled Keywords: Ananas comosus, Fruit bromelain, Fruit ripening, MD 2, Pest resistance, qPCR analysis
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Science
Kulliyyah of Science > Department of Biotechnology
Depositing User: AP. Dr. Azzmer Azzar Abdul Hamid
Date Deposited: 19 Jan 2021 11:40
Last Modified: 19 Jan 2021 11:47
URI: http://irep.iium.edu.my/id/eprint/82591

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