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Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis

Bakar, Nur Husnina Iffah and Lee, Thong Chuan and Mohd-Hairul, Ab Rahim and Ahmad Raus, Raha and Basri, Dayang Fredalina and Adzahar, Noor Suhana (2020) Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis. In: Materials Science Forum. Trans Tech Publications Ltd, Kuantan, Malaysia, pp. 265-270. ISBN 978-303571664-1

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A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL. The phytochemical compounds in the extract were analyzed by using the Gas Chromatography-Mass Spectrometry (GC-MS). The GC-MS result showed that the extract contains bioactive compounds such as n-hexadecanoic acid, pentadecanoic acid, phenol, 2,4-bis(1,1- dimethylethyl) and octadecanoic acid that have bioactive properties such as cytotoxic and antioxidant. The cytotoxic effect of the extract on normal cell line was assessed by using Real-Time Cell Analysis (RTCA) instrument. The result showed that there was no significant cytotoxicity effect against Vero cell lines. This result showed that the real-time impedance-based assay can be utilized to monitor changes of the cells and to determine the inhibitory concentration of the extract.

Item Type: Book Chapter
Additional Information: 3911/82587
Uncontrolled Keywords: Cytotoxicity, Hibiscus, RTCA, Vero cell
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering
Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Raha Ahmad Raus
Date Deposited: 02 Sep 2020 09:30
Last Modified: 02 Sep 2020 15:55
URI: http://irep.iium.edu.my/id/eprint/82587

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