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Bioactive compounds from Aspergillus niger extract enhance the antioxidant activity and prevent the genotoxicity in aflatoxin B1-treated rats

Abdel-Wahhab, Mosaad Attia and El-Nekeety, Aziza Abdel Salam and Hathout, Amal S. and Salman, Asmaa S. and Abdel-Aziem, Sekena H. and Sabry, Bassem A. and Hassan, Nabila Salah and Abdel-Aziz, Mohammed S. and Aly, Soher E. and Jaswir, Irwandi (2020) Bioactive compounds from Aspergillus niger extract enhance the antioxidant activity and prevent the genotoxicity in aflatoxin B1-treated rats. Toxicon, 181. pp. 57-68. ISSN 0041-0101 E-ISSN 1879-3150

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Abstract

This study aimed to identify the bioactive compounds of the ethyl acetate extract of Aspergillus niger SH2-EGY using GC-MS and to evaluate their protective role against aflatoxin B1 (AFB1)-induced oxidative stress, genotoxicity and cytotoxicity in rats. Six groups of male Sprague-Dawley rats were treated orally for 4 weeks included the control group, AFB1-treated group (80 μg/kg b.w); fungal extract (FE)-treated groups at low (140) or high dose (280) mg/kg b.w and the groups treated with AFB1 plus FE at the two tested doses. The GC-MS analysis identified 26 compounds. The major compounds found were 1,2,3,4,6-Penta-trimethylsilyl Glucopyranose, Fmoc-L-3-(2-Naphthyl)-alanine, D-(-)-Fructopyranose, pentakis (trimethylsilyl) ether, bis (2-ethylhexyl) phthalate, trimethylsilyl ether-glucitol, and octadecanamide, N-(2- methylpropyl)-N-nitroso. The in vivo results showed that AFB1 significantly increased serum ALT, AST, creatinine, uric acid, urea, cholesterol, triglycerides, LDL, carcinoembryonic antigen, alpha-fetoprotein, interleukin-6, Malondialdehyde, nitric oxide, Bax, caspase-3 and P53 mRNA expression, chromosomal aberrations and DNA fragmentation. It decreased serum TP, albumin, HDL, Bcl-2 mRNA expression, hepatic and renal TAC, SOD and GPx content and induced histological changes in the liver and kidney. FE prevented these disturbances in a dosage-dependent manner. It could be concluded that A. niger SH2-EGY extract is safe a promising agent for pharmaceutical and food industries.

Item Type: Article (Journal)
Additional Information: 5784/82209
Uncontrolled Keywords: Aflatoxin B1, Aspergillus niger, Bioactive compounds, Cytotoxicity, Genotoxicity, Oxidative stress
Subjects: Q Science > QH Natural history > QH301 Biology
T Technology > TP Chemical technology
T Technology > TP Chemical technology > TP368 Food processing and manufacture
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): International Institute for Halal Research and Training (INHART)
Depositing User: Dr Irwandi Jaswir
Date Deposited: 17 Aug 2020 09:19
Last Modified: 06 Oct 2020 15:30
URI: http://irep.iium.edu.my/id/eprint/82209

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