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Polyamidoamine dendrimers: favorable polymeric nanomaterials for lipase activation

Elgharbawy, Amal A.M. and Syed Putra, Sharifah Shahira and Hayyan, Maan and Hayyan, Adeeb and Basirun, Wan Jefrey and Mirghani, Mohamed Elwathig Saeed and Wan Nawawi, Wan Mohd Fazli and Majrashi, Abdulrahman Abdulaziz and Alias, Yatimah and Mohd. Salleh, Hamzah and Tok, Tugba Taskin (2020) Polyamidoamine dendrimers: favorable polymeric nanomaterials for lipase activation. Materials Today Communications, 26. p. 101492. ISSN 2352-4928 (In Press)

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Abstract

Dendrimers are highly branched synthetic macromolecules that offer promising prospects for utilizing them in broad applications. This study investigates the role of polyamidoamine (PAMAM) dendrimer for lipase-catalyzed reaction in aqueous solution. The hydrolysis of para-nitrophenyl palmitate (pNPP) by four different lipases was tested with PAMAM dendrimer with different generations (i.e., D0.0, D2.5, D3.0, and D4.0). The results showed that the activity of Rhizopus niveus lipase (RNL) was enhanced up to 375% with PAMAM dendrimer (D0.0). The optimum incubation conditions such as the temperature and coupling time of the lipase with D0.0 were 40 °C and 2 h, respectively. The behavior of lipases at different pH showed that RNL was mostly active at pH 7.0. Most lipases with D0.0 were enhanced at least two-fold compared to the control samples. Kinetic study showed that the RNL affinity was enhanced in the presence of PAMAM. FTIR spectra strongly suggest that hydrogen bonding and electrostatic interactions were the main types of bonding in the RNL and PAMAM system. Additionally, the molecular docking simulation of the system was exerted to evaluate and support the related compound, (i.e., D0.0) as a ligand interacted effectively with RNL based on the experimental activity. The cytotoxicity study revealed mild toxicity of the system which could serve as a potential target in nanomedicine for many applications such as cancer therapeutics.

Item Type: Article (Journal)
Additional Information: 8637/81987
Uncontrolled Keywords: Rhizopusniveuslipase natural deep eutectic solvent ionic liquid drug delivery nanotechnology nanomedicine molecular docking simulation
Subjects: Q Science > Q Science (General)
T Technology > T Technology (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): International Institute for Halal Research and Training (INHART)
Kulliyyah of Engineering
Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: A Prof Elwathig Mirghani
Date Deposited: 10 Aug 2020 11:39
Last Modified: 10 Aug 2020 11:40
URI: http://irep.iium.edu.my/id/eprint/81987

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