Effects of carbon sources, plant growth regulators and inoculum size on Citrus suhuiensis cell suspension culture growth

Mohamad Puad, Noor Illi and M. Fathil, Nur Alia and Amid, Azura and Azmi, Azlin Suhaida (2020) Effects of carbon sources, plant growth regulators and inoculum size on Citrus suhuiensis cell suspension culture growth. Malaysia Applied Biology Journal, 49 (1). pp. 87-93. ISSN 0126-8643

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Abstract

Cell suspension culture of Citrus suhuiensis (C. suhuiensis) was established to determine the best growth condition based on the effects of carbon source, plant growth regulators (PGRs) and inoculum size. Friable callus from the cotyledon explant was used to initiate the cell suspension cultures. Murashige and Skoog (MS) medium supplemented with 50 g/L glucose achieved the highest cell dry weight (CDW) and μmax of 23.2 g/L and 0.47 h-1 respectively compared to glucose at 30 g/L and other carbon sources. For PGRs effects, 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) gave the highest amount of CDW and μmax; 11.91 g/L and 0.32 h-1. Furthermore, the inoculum size at 10% (v/v) gave the maximum CDW and μmax of 15.45 g/L and 0.71 h-1 respectively compared to 20% (v/v) and 30% (v/v) of inoculum. The results achieved can be used for further statistical optimization of C. suhuiensis cell suspension culture growth parameters in which the interactions between those parameters can be further studied and understood.

Item Type:	Article (Journal)
Additional Information:	5462/81881
Uncontrolled Keywords:	Citrus suhuiensis, cell suspension culture, carbon source, inoculum size, plant growth regulators (PGRs).
Subjects:	S Agriculture > SB Plant culture T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART) Kulliyyah of Engineering Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Noor Illi Mohamad Puad
Date Deposited:	03 Aug 2020 12:44
Last Modified:	03 Aug 2020 12:44
URI:	http://irep.iium.edu.my/id/eprint/81881

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