Amniotic membrane enhance the effect of vascular endothelial growth factor on the angiogenic marker expression of stem cells from human exfoliated deciduous teeth

Yusof, Muhammad Fuad Hilmi and Md Hashim, Siti Nurnasihah and Zahari, Wafa’ and Chandra, Hamshawagini and Ahmad Amin Noordin, Khairul Bariah and Thirumulu Ponnuraj, Kannan and Sheikh Ab. Hamid, Suzina and Mokhtar@Makhtar, Khairani Idah and Ahmad, Azlina (2020) Amniotic membrane enhance the effect of vascular endothelial growth factor on the angiogenic marker expression of stem cells from human exfoliated deciduous teeth. Applied Biochemistry and Biotechnology, 191. pp. 177-190. ISSN 0273-2289 E-ISSN 1559-0291

	PDF - Published Version Restricted to Registered users only Download (2MB) | Request a copy
Preview	PDF (SCOPUS) - Supplemental Material Download (248kB) | Preview
Preview	PDF (WOS) - Supplemental Material Download (285kB) | Preview

Abstract

Previously, it was reported that human amniotic membrane (AM) induced stem cells from human deciduous exfoliated teeth (SHED) endothelial-like-cell differentiation. This interesting effect of AM matrix on SHED demands further elucidation. Objective of this in vitro work was to study the effect of 24-h VEGF induced on SHED endothelial differentiation when seeded on acellular stromal side (SS) of AM matrix. Stemness of SHED was identified by flow cytometry. Cell attachment and morphological changes towards the matrix was observed by scanning electron microscopy. Protein expression of endothelial marker was examined by Western blot. The expression of stem cells and endothelial-specific gene markers of VEGF-induced SHED cultured on human AM was inspected via reverse transcriptase-polymerase chain reaction. Results showed SHED at both passages retain stemness property. Ang-1 protein was expressed in SHED. Cells treated with VEGF and cultured on AM transformed attached well to AM. VEGF induced SHED expressed both stem cell and endothelial-specific markers throughout the treatments and timeline. Interestingly, prolonged VEGF treatment increased the expression of Cox-2 and VE-Cadherin genes in all treated groups when compared to SHED. It was concluded that the VEGF-induced SHED showed better expression of endothelial-specific markers when cultured on SS of AM, with prolonged VEGF treatment.

Item Type:	Article (Journal)
Additional Information:	7172/81074
Uncontrolled Keywords:	SHED . Human amniotic membrane . VEGF. Angiogenic differentiation . Tissue engineering
Subjects:	R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences Kulliyyah of Dentistry
Depositing User:	Assoc Prof Khairani Idah Mokhtar
Date Deposited:	27 Jul 2020 14:55
Last Modified:	27 Jul 2020 14:55
URI:	http://irep.iium.edu.my/id/eprint/81074

Actions (login required)

View Item