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Characterisation of osteomyelitis microbiome using specific growth media

Ali, Fatimah Maryam and Ismail, Mohd Faisal and Mohktar, Munirah and Rismayuddin, Alia Risma and Yusof, Mohd Nazri and Arzmi, Mohd Hafiz (2019) Characterisation of osteomyelitis microbiome using specific growth media. International Journal of Allied Health Sciences, 3 (3 (Special Issue: Research Towards Sustainable Development Goals) Kuantan Research Day 2019). p. 757. E-ISSN 2600-8491

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Osteomyelitis is an inflammation of the bone caused by microbial infection. The objective of the study is to characterise the microbiome from osteomyelitis with the hypothesis that osteomyelitis is induced by polymicrobial infection. To identify the microbiome, sample was initially isolated from pin site of ortophaedic patients with osteomyelitis using sterile swab. Following that, the sample was inoculated into nutrient broth and incubated at 37° C for 24 h aerobically. Later, the sample was cultured on CHROMagar, mitis salivarius agar (MSA) and lactobacillus specific agar (LSA) for identification of Candida species, Streptococcus species and Lactobacillus species, respectively. Gram-staining was conducted to identify its phenotype under light microscope. Finally, disc diffusion test was conducted on each isolate to assess its sensitivity towards nystatin, amphotericin B, gentamicin and tetracycline. Based on the study, only Candida albicans and Streptococcus species were identified. Candida albicans and Streptococcus species were isolated from the sample with C. albicans exhibited resistance to amphotericin B, gentamicin and tetracycline while Streptococcus species were resistance to nystatin, amphotericin B and tetracyclin. In conclusion, osteomyelitis is induced by polymicrobial infection, with the microbial isolates possess various susceptibility towards common antimicrobial agents.

Item Type: Article (Meeting Abstract)
Additional Information: 6888/80862
Uncontrolled Keywords: Osteomyelitis, polymicrobial infection, disc diffusion assay
Subjects: Q Science > QR Microbiology
R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Dentistry
Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences
Depositing User: Dr Mohd Hafiz Arzmi
Date Deposited: 30 Jun 2020 08:58
Last Modified: 30 Jun 2020 09:29
URI: http://irep.iium.edu.my/id/eprint/80862

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