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Assessment of Free radical scavenging and digestive enzyme inhibitory activities of extract, fractions and isolated compounds from Tetracera macrophylla leaves

Opeyemi Roheem, Fatimah and Ahmed, Qamar Uddin and Mat So'ad, Siti Zaiton and Ali Shah, Syed Adnan and Latip, Jalifah and Alhassan, Alhassan Muhammad and Syed Mohammad, Sharifah Nurul Akilah (2020) Assessment of Free radical scavenging and digestive enzyme inhibitory activities of extract, fractions and isolated compounds from Tetracera macrophylla leaves. Journal of Herbal Medicine, xx (xx). pp. 1-8. ISSN 2210-8033 (In Press)

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Abstract

Tetracera macrophylla is a valuable medicinal plant used traditionally for the treatment of various ailments. The crude ethanol extract was analyzed for its total phenolic and flavonoid contents. 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-3-ethylbenzothiazine-6-sulfonic acid (ABTS) were used to examine the free radical scavenging capacity while enzyme inhibitory activity was assessed using α-amylase and α-glucosidase inhibitory methods. The crude extract was found to be rich in both phenolic and flavonoid contents (417.90 and5.86 μg GAE and QUE/mg dry weight, respectively) thus exhibited good radical scavenging activity on DPPH and ABTS radicals (IC50 values of 16.2 and 14.65 μg/mL, respectively). It also showed a promising concentration dependent enzymes inhibitory activity against α-amylase and α-glucosidase with IC50 of 72.17 and 76.16 μg/mL, respectively. Fractionation of active ethanol extract of T. macrophylla leaves through liquid-liquid partitioning yielded fractions Fa, Fb, Fc, and Fd. Among all the fractions, Fb (ethyl acetate fraction) showed the highest phenolic and flavonoid contents (206.34 μg/ GAE and 4.01 μg/ QUE respectively) and was also found to exhibit highest antioxidant and α-glucosidase inhibitory activities. The most active fraction, Fb upon purification yielded five compounds which were characterized and identified through different spectroscopic techniques. FTQ-4 among the compounds exhibited the strongest free radical and enzyme inhibitory activities followed by FTQ-3. All the compounds were isolated from this plant for the first time and found active against free radicals and/or digestive enzymes.These results further suggest the medicinal potential of T. macrophylla leaves as a therapeutic agent against hyperglycemia and free radical associated problems.

Item Type: Article (Journal)
Additional Information: 4921/80752
Uncontrolled Keywords: Tetracera macrophylla, DPPH, ABTS, α-glucosidase α-amylase, Active principles,
Subjects: Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Kulliyyah of Pharmacy
Depositing User: Dr. Siti Zaiton Mat So'ad
Date Deposited: 18 Jun 2020 12:15
Last Modified: 18 Jun 2020 12:15
URI: http://irep.iium.edu.my/id/eprint/80752

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