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Gum Arabic more than emulsifier and food additive: a new experimental validation of anti-proliferative against colorectal colon cancer cell lines

Elnour, Ahmed Adam Hassan M. and Mirghani, Mohamed Elwathig Saeed and Alam, Md Zahangir and Daddiouaissa, Djabir and Kabbashi, Nassereldeen Ahmed and Musa, Khalid Hamid and Nour, Abdurahman Hamid (2020) Gum Arabic more than emulsifier and food additive: a new experimental validation of anti-proliferative against colorectal colon cancer cell lines. International Journal of Food, Nutrition and Public Health (IJFNPH), 11 (1/2 Part 2). pp. 83-95. ISSN 2042-5988 E-ISSN 2042-5996

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Abstract

The aim of this paper is to discover the cytotoxicity activity of Acacia seyal gum and Prebio-Tcommercial (PTC) samples on human colon cancer (HCT-116) cell lines; in addition, its polyphenolic properties are investigated. Approach: The methodology used in this paper involves fractionating methanol crude extracts of both Acacia seyal gum and Prebio-T-commercial, respectively. The cytotoxicity activity on human colon cancer cell lines for each fraction was studied using sulforhodamine B assay (SRB assay). Both samples, Acacia seyal gum and Prebio-T-commercial (PTC), were analysed precisely using high performance liquid chromatography (HPLC) technique. Findings: Based on the analysis, more cytotoxicity activity was identified in the sample obtained from Acacia seyal gum (IC50=13.36µg/mL) compared to PTC (IC50=17.18µg/mL). Regarding the flavonoids content (FC), quercetin was the only flavonoids detected in the samples, found to be approximate of 4,640µg/100g for Acacia seyal gum, almost double the value of 2,560µg/100g for PTC. Implications: The implications of this study are the evidence of flavonoids content and anti-cancer activity for both Acacia seyal gum and PTC used in folk medicine, as well as anticipation of cancer cell growth. It is expected that the proposed methods will give a noble contribution to the possible use of their bioactive compounds as natural anti-cancer agents.

Item Type: Article (Journal)
Additional Information: 4971/79962
Uncontrolled Keywords: Cytotoxicity activity; Acacia seyal gum; Colorectal cancer (HCT-116); Flavonoids, and HPLC.
Subjects: Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): International Institute for Halal Research and Training (INHART)
Kulliyyah of Engineering
Depositing User: A Prof Elwathig Mirghani
Date Deposited: 17 Apr 2020 14:38
Last Modified: 17 Apr 2020 14:40
URI: http://irep.iium.edu.my/id/eprint/79962

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