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Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compaounds in the leaves of Vitex Trifolia linn processing anti-tracheospasmolytic activity

Khatib, Alfi and Hoek, Arie C. and Jinap, Selamat and Sarker, Md. Zaidul Islam and Jaswir, Irwandi and Verpoorte, Robert (2010) Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compaounds in the leaves of Vitex Trifolia linn processing anti-tracheospasmolytic activity. Journal of Liquid Chromatography & Related Technologies, 33. pp. 214-224. ISSN 1082-6076 print/1520-572X online

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Abstract

We have developed one approach to fingerprint and estimate the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic assay using two-dimensional TLC pattern comparison. Based on the two-dimensional TLC pattern and the activity of the centrifugal partition chromatography fractions, we concluded that the semi polar compounds were responsible for anti-tracheospasmolytic activity. The best non-polar=semi polar mobile phases for the two-dimensional TLC using silica gel as the stationary phase were chloroform=methanol (9=1) as the first mobile phase and ethyl cetate=chloroform=methanol (28=28=44) as the second mobile phase.

Item Type: Article (Journal)
Additional Information: 5784/7962
Uncontrolled Keywords: Asthma, centrifugal partition chromatography, fingerprinting, thin layer chromatography, tracheospasmolytic assay, Vitex trifolia Linn
Subjects: Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Irwandi Jaswir
Date Deposited: 30 Nov 2011 12:38
Last Modified: 16 Dec 2013 13:02
URI: http://irep.iium.edu.my/id/eprint/7962

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