Optimization of bio-active compounds from stem of Anisophyllea disticha using supercritical fluid extraction technique

Ferdosh, Sahena and Abdul Bari, Nurul Ashikin and Mia, Abdur Rashid and Sarker, Md. Zaidul Islam (2019) Optimization of bio-active compounds from stem of Anisophyllea disticha using supercritical fluid extraction technique. In: AIMST International Pharmacy Conference (AIPC) 2019: Challenges and Opportunities in Pharmaceutical Sciences, Technology and Practice, 30 November - 1 December 2019, Faculty of Pharmacy, AIMST University, Semeling, Kedah. (Unpublished)

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Abstract

Background: Anisophyllea disticha is belonging to the species of genus Anisophyllea, is not pharmacologically investigated despite its high utilization by the folklore in treating diarrhea, dysentery, jaundice, revitalizing the birth canal of women, relieving weariness and body aches. Objective: To optimize the total phenolic content (TPC) and antioxidant activities of supercritical fluid extracts of A. disticha in terms of DPPH radical scavenging activities and ferric reducing antioxidant power assay. Material and methods: Stem was chosen and subsequently extracted using supercritical fluid extraction (SFE) and further analyzed. Box-Behnken Design (BBD) was used to optimize and examine the effect of independent variables of SFE such as temperature (50-70˚C), pressure (20-30 MPa) and concentration of co-solvent (10-20%) on TPC and antioxidant activities of A. disticha. Results: The model suggested the optimal conditions of independent variables to be 50˚C, 20 MPa and 17% with predicted TPC of 53.35 mg GAE / g extract, scavenging activity of 96.61% and reducing power of 423.66 mg Fe (II) / g extract. Reverse-phase high performance liquid chromatography (HPLC) was developed for identification and quantification of phenolic compounds (gallic acid, p-coumaric, ferulic acid and quercetin) presented in A. disticha. Run 7 from SFE was chosen as it showed maximum TPC and potent activities and the responses obtained were compared with soxhlet Conclusion: The extract from SFE possessed remarkable TPC, antioxidant activities and concentration of phenolic compounds identified by HPLC, indicating its superior recovery of compounds.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	6605/79158
Uncontrolled Keywords:	Anisophyllea disticha; supercritical fluid extraction; antioxidant
Subjects:	R Medicine > RS Pharmacy and materia medica > RS192 Materia Medica-Pharmaceutical Technology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Technology Kulliyyah of Science Kulliyyah of Science > Department of Plant Science
Depositing User:	Professor Md. Zaidul Islam Sarker
Date Deposited:	02 Mar 2020 08:29
Last Modified:	02 Mar 2020 08:29
URI:	http://irep.iium.edu.my/id/eprint/79158

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