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Antibacterial activity of ethanol extract of Sargassum polycystum against Streptococcus mutans and Lactobacillus casei: in vitro

Ab. Halim, Noorhazayti and Zakaria, Nurul Syahidah and Md Hisham, Sarah Shafinaz and Husain, Juzaily and Md Mustafa, Md Muziman Syah (2020) Antibacterial activity of ethanol extract of Sargassum polycystum against Streptococcus mutans and Lactobacillus casei: in vitro. International Journal of Allied Health Sciences, 4 (1). pp. 1121-1127. E-ISSN 2600-8491

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Abstract

Introduction: Sargassum polycystum (brown seaweeds) has been recognized as a potential source of natural antibacterial and antimicrobial compounds. It has been used in many applications and products as they possess interesting biological activities that give benefit to the dental and medical area. Aims: The objectives of this study were to determine the growth curve of Streptococcus mutans and Lactobacillus casei, and also to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) value of Sargassum polycystum extract against Streptococcus mutans and Lactobacillus casei. Materials and methods: The growth of both bacteria were studied by growth curve analysis. Besides that, the antibacterial activity of Sargassum polycystum ethanol extracts against two oral cariogenic bacteria were examined using broth dilution method. The bioactivity of the seaweed extract was studied by finding the MIC and MBC value. Results: The time taken for the Streptococcus mutans and Lactobacillus casei to achieve exponential phase during growth is 4 and 5 hours respectively. MIC values of ethanol extracts of Sargassum polycystum in different concentration against Streptococcus mutans and Lactobacillus casei were unreliable because the presence of fungus (white-precipitate) at all 96-well plates including the positive and negative control. As there was no MIC value, partial inhibition was determined by calculating the optical density. Therefore, MBC analysis was unable to carry out because the MIC values were unreliable. Conclusion: The ethanol extract of Sargassum polycystum used in this study show partial bacterial inhibition against pathogens used. Therefore, the same study by using different type of solvent are recommended to be carried out in the future.

Item Type: Article (Journal)
Additional Information: 5869/79022
Uncontrolled Keywords: Sargassum polycystum, brown seaweed, antibacterial activity, Streptococcus mutans, Lactobacillus casei, growth curve
Subjects: R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences > Department of Optometry and Visual Science
Kulliyyah of Dentistry > Paediatric Dentistry, Orthodontics & Dental Public Health
Kulliyyah of Dentistry > Restorative Dentistry
Depositing User: Dr. Md Muziman Syah Md Mustafa
Date Deposited: 23 Feb 2020 02:04
Last Modified: 26 Feb 2020 23:00
URI: http://irep.iium.edu.my/id/eprint/79022

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