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Rapid method for determining malondialdehyde as secondary oxidation product in palm olein system by Fourier transform infrared spectroscopy

Mirghani, Mohamed Elwathig Saeed and Che Man, Yakoob and Selamat, Jinap and Baharin , B. S. and Bakar, Jamilah (2002) Rapid method for determining malondialdehyde as secondary oxidation product in palm olein system by Fourier transform infrared spectroscopy. Phytochemical Analysis , 13 (4). pp. 195-201. ISSN 1099-1565

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Abstract

A simple and rapid Fourier transform infrared (FTIR) spectroscopic method has been developed for the quantitative determination of malondialdehyde as secondary oxidation product in a palm olein system. The FTIR method was based on a sodium chloride transmission cell and utilised a partial least square statistical approach to derive a calibration model. The frequency region combinations that gave good calibration were 2900–2800, and 1800–1600 cm−1. The precision and accuracy, in the range 0–60 µmol malondialdehyde/kg oil, were comparable to those of the modified distillation method with a coefficient of determination (r2) of 0.9891 and standard error of calibration of 1.49. The calibration was cross-validated and produced an r2 of 0.9786 and standard error of prediction of 2.136. The results showed that the FTIR method is versatile, efficient and accurate, and suitable for routine quality control analysis with the result obtainable in about 2 min from a sample of less than 2 mL. Copyright © 2002 John Wiley & Sons, Ltd.

Item Type: Article (Journal)
Additional Information: ISI Journal Citation Reports®:2010:Biochemical Research Methods: 45/71; Chemistry, Analytical:34/71; Plant Sciences:64/187; (Impact Factor: 1.848) 4971/7790
Uncontrolled Keywords: FTIR spectroscopy, malondialdehyde, palm olein system, partial least square, thiobarbituric acid.
Subjects: T Technology > T Technology (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Assoc Prof Elwathig Mirghani
Date Deposited: 08 May 2012 08:43
Last Modified: 08 May 2012 08:43
URI: http://irep.iium.edu.my/id/eprint/7790

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