Measurement of pterygium tissue dry weight using two different tissue preparation techniques in freeze-dry method

Hilmi, Mohd Radzi and Mohd Kamal, Khairidzan and Che Azemin, Mohd Zulfaezal and Ariffin, Azrin Esmady (2019) Measurement of pterygium tissue dry weight using two different tissue preparation techniques in freeze-dry method. Sains Medika. ISSN 2085-1545 E-ISSN 2339-093X (In Press)

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Abstract

Introduction: The proposed freeze-dried approach for estimating pterygium dry weight closes the gap which address the possible effect of corneal compression due to pterygium on the corneal surface, translating simple laboratory procedure to a clinical practice which essential in predicting induced-corneal astigmatism. This study aim to compare an objective method in quantifying pterygium dry weight utilizing freeze-dried method via two different tissue preparation methods. Methods: 60 primary pterygium were excised using controlled partial avulsion technique performed by a single surgeon (KMK), and divided into two groups: formalin-fixed (n=30) and non-fixed (n=30). Initially, each sterile container weight was measured and 5ml of 5% buffered formaldehyde were filled and stored for 1 week for formalin-fixed group while, 5ml distilled water were filled for non-fixed pterygium group. Each container was pre-frozen for 12 hours prior to freeze-dried (- 80 ºC for 24 hours). The final product known as net pterygium tissue mass (NPTM) then weighted as dry weight. Comparative analysis of wet and dry weight, and percentage of NPTM between groups were performed via independent t-test. Results: The overall mean and SD for formalin-fixed and non-fixed pterygium wet weight were 253.33 ± 82.17 μg and 255.17 ± 63.52 μg, and dry weight were 184.92 ± 84.31 μg and 179.54 ± 72.85 μg respectively. In terms of percentage of NPTM, formalin-fixed group revealed slightly higher percentage compared to non-fixed pterygium tissue with 69.39 ± 13.29 % and 67.75 ± 13.27 % respectively. The difference of pterygium dry weight, between formalin-fixed and non-fixed pterygium tissue were statistically insignificance (P = 0.792). Conclusions: Freeze-dried method can be used to quantify the NPTM (dry weight) of pterygium fibrovascular tissue. Both methods (formalin-fixed and non-fixed) are reliable in producing results. This is proven as there is no statistical significance between the two methods.

Item Type:	Article (Journal)
Additional Information:	7746/77596
Uncontrolled Keywords:	Pterygium; freeze-dried; dry weight; net pterygium tissue mass; corneal astigmatism
Subjects:	R Medicine > RE Ophthalmology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Optometry and Visual Science Kulliyyah of Medicine > Department of Ophthalmology Kulliyyah of Allied Health Sciences Kulliyyah of Medicine
Depositing User:	Dr Mohd Radzi Hilmi
Date Deposited:	28 Jan 2020 13:20
Last Modified:	16 Jun 2022 16:32
URI:	http://irep.iium.edu.my/id/eprint/77596

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