Environmental factors associated with the presence of vibrionaceae in tropical cage-cultured marine fishes

Ahmad, Nurliyana and Amal, Mohammad Noor Azmai and Saad, mohd zamri and Md Yasinb, Ina Salwany and Al-saari, Nurhidayu (2019) Environmental factors associated with the presence of vibrionaceae in tropical cage-cultured marine fishes. Journal of Aquatic Animal Health, 31. pp. 154-167. ISSN 0899-7659 E-ISSN 1548-8667

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Abstract

This study investigated the environmental factors associated with the presence of Vibrionaceae in economically important cage-cultured tropical marine fishes: the Asian Seabass Lates calcarifer, snapper Lutjanus sp., and hybrid grouper Epinephelus sp. Fish sampling was conducted at monthly intervals between December 2016 and August 2017. The body weight and length of individual fish were measured, and the skin, eye, liver, and kidney were sampled for bacterial isolation and identification. Water physicochemical parameters during the sampling activities were determined, and the enumeration of total Vibrionaceae count was also conducted from water and sediment samples. Nine species of Vibrio were identified, including V. alginolyticus, V. diabolicus, V. harveyi, V. campbellii, V. parahaemolyticus, V. rotiferianus, V. furnissii, V. fluvialis, and V. vulnificus. Photobacterium damselae subsp. damselae was also identified. A total of 73% of the isolated Vibrio belonged to the Harveyi clade, followed by the Vulnificus clade (5.5%) and Cholera clade (0.6%). Highest occurrence of Vibrio spp. and P. damselae subsp. damselae was found in hybrid grouper (72%), followed by Asian Seabass (48%) and snapper (36%). The associations of Vibrio spp. and P. damselae subsp. damselae with the host fish were not species specific. However, fish mortality and fish size showed strong associations with the presence of some Vibrio spp. On average, 60% of the infected cultured fish exhibited at least one clinical sign. Nevertheless, inconsistent associations were observed between the pathogens and water quality. The yearlong occurrence and abundance of Vibrionaceae in the environmental components indicate that they might serve as reservoirs of these pathogens.

Item Type:	Article (Journal)
Additional Information:	8649/75661
Uncontrolled Keywords:	Environmental factors associated; Vibrionaceae in tropical; Cage-cultured marine fishes
Subjects:	Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART)
Depositing User:	Dr Nurhidayu al-saari
Date Deposited:	08 Jan 2020 11:43
Last Modified:	24 Jan 2020 08:50
URI:	http://irep.iium.edu.my/id/eprint/75661

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