Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED)

Nordin, Nur Sazwi and Mokhtar@Makhtar, Khairani Idah and Mustafa Al-Ahmad, Basma Ezzat and Lestari, Widya and Arief Ichwan, Solachuddin Jauhari and Darnis, Deny Susanti and Thirumulu Ponnuraj, Kannan and Ahmad, Azlina (2019) Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED). In: International Conference on Pharmaceutical Research and Pharmacy Practice cum 14th IIUM-MPS Pharmacy Scientific Conference (ICPRP 2019), 19-20 October 2019, Istana Hotel, Kuala lumpur. (Unpublished)

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Abstract

Introduction: Flaxseed extract promotes bone health and possibly function in inducing bone regeneration process. SHED have been considered as an alternative source of MSC in tissue engineering because of their potential to differentiate into multiple cell-lineages including osteoblasts. Although flaxseed extract has been shown to affect bone formation process, its effect in inducing stem cell into osteoblast lineage has not been reported. Objective: To determine the effects of flaxseed extract on osteoblast differentiation potential of SHED. Materials and methods: SHED cultured in osteoblast induction media (OIM) was treated with flaxseed extract at 4mg/ml. The RNA was collected and extracted with Total RNA Mini Kit (Geneaid) from cells cultured at day 1, 3, 7, 14 and 21 and subjected to reverse-transcriptase PCR for osteoblast markers (OSX, OCN, DMP1). Alkaline phosphatase activity was determined by ALP assay and Alizarin Red S staining was performed to evaluate calcium deposits in SHED. Results: All osteoblast markers were expressed in all samples analysed. Interestingly, the expression of OSX appeared to be reduced in SHED treated with flaxseed extract. In addition, SHED treated with flaxseed extract resulted in low ALP activity compared to control (p<0.05) (t-test). Calcium deposition was positive in SHED cultured in OIM only. Conclusion: Flaxseed appears to reduce the expression of osteoblast markers, ALP activity and calcium deposition in SHED. This suggests that flaxseed as a potential inhibitor in osteoblast differentiation of SHED.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	7172/75491
Uncontrolled Keywords:	SHED, flaxseed extract, osteoblast differentiation
Subjects:	R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences Kulliyyah of Science > Department of Chemistry
Depositing User:	Assoc Prof Khairani Idah Mokhtar
Date Deposited:	18 Nov 2019 12:50
Last Modified:	18 Nov 2019 12:50
URI:	http://irep.iium.edu.my/id/eprint/75491

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